Pound A W, Lawson T A, Horn L
Br J Cancer. 1973 Jun;27(6):451-9. doi: 10.1038/bjc.1973.57.
Rats were given a single dose of dimethylnitrosamine (DMN, 20 mg/kg body weight) alone or 42 or 60 hours after a non-lethal hepatotoxic dose of carbon tetrachloride (CC1(4)) and killed 12 months later. DMN alone produced no tumours in the kidney and a few in the liver, but when given 42 hours after CC1(4), tumours formed in the kidneys and the number in the liver was increased. When given after 60 hours, the incidence of kidney tumours was less but that of liver tumours was further increased. A larger dose of DMN (40 mg/kg) was tolerated 42 hours after CC1(4) and enhanced the number of kidney and liver tumours, the latter apparently due to an increased proportion of cholangiomata. Numerous small focal proliferations of atypical liver cells and of bile duct epithelium were observed after treatment with DMN. The incidence of these lesions in the different experimental treatments varied in a similar manner to the liver tumours.
给大鼠单独注射一次剂量的二甲基亚硝胺（DMN，20毫克/千克体重），或者在给予非致死性肝毒性剂量的四氯化碳（CC1(4)）42或60小时后注射，12个月后处死大鼠。单独注射DMN时，肾脏未产生肿瘤，肝脏仅有少数肿瘤，但在CC1(4)注射42小时后注射DMN，肾脏出现肿瘤，肝脏肿瘤数量增加。在60小时后注射，肾脏肿瘤的发生率较低，但肝脏肿瘤的发生率进一步增加。CC1(4)注射42小时后，大鼠能耐受更大剂量的DMN（40毫克/千克），肾脏和肝脏肿瘤数量增加，后者显然是由于胆管瘤比例增加。用DMN治疗后，观察到大量非典型肝细胞和胆管上皮的小灶性增生。这些病变在不同实验处理中的发生率与肝脏肿瘤的变化方式相似。
