大肠杆菌DNA依赖性和非依赖性核糖核苷三磷酸酶活性与dnaB基因产物的关联。
Association of DNA-dependent and -independent ribonucleoside triphosphatase activities with dnaB gene product of Escherichia coli.
作者信息
Wickner S, Wright M, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):783-7. doi: 10.1073/pnas.71.3.783.
Abstract
Preparations of E. coli dnaB gene product contain ribonucleoside triphosphatase activity that is stimulated 10-fold by DNA. The products of the triphosphatase activity are nucleoside diphosphates and P(i). The dnaB complementing activity in the varphiX174 DNA-dependent system and these triphosphatase activities copurify over the last 20-fold of an extensive (about 40,000-fold) purification procedure. Acrylamide gel electrophoresis of the purified material shows a single band of protein coincident with eluted dnaB complementing and DNA-dependent and -independent nucleoside triphosphatase activities.
摘要
大肠杆菌dnaB基因产物的制剂含有核糖核苷三磷酸酶活性，该活性可被DNA刺激10倍。三磷酸酶活性的产物是核苷二磷酸和无机磷酸（Pi）。在φX174 DNA依赖性系统中的dnaB互补活性和这些三磷酸酶活性在一个广泛的（约40000倍）纯化过程的最后20倍纯化中共同纯化。纯化材料的丙烯酰胺凝胶电泳显示一条单一的蛋白带，与洗脱的dnaB互补活性以及依赖和不依赖DNA的核苷三磷酸酶活性一致。
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Association of DNA-dependent and -independent ribonucleoside triphosphatase activities with dnaB gene product of Escherichia coli.大肠杆菌DNA依赖性和非依赖性核糖核苷三磷酸酶活性与dnaB基因产物的关联。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):783-7. doi: 10.1073/pnas.71.3.783.
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