Stringfellow D A, Glasgow L A
Infect Immun. 1974 Dec;10(6):1337-42. doi: 10.1128/iai.10.6.1337-1342.1974.
As previously reported, mice infected with encephalomyocarditis (EMC) virus progressively develop a state of hyporeactivity to interferon induction as evidenced by an 80% reduction in serum interferon levels produced in response to four different inducers administered 96 h after initiation of infection. In the current report, this hyporesponsiveness was further documented in vitro in peritoneal exudate cells harvested at the time of maximal hyporeactivity (96 h) during EMC virus infection. These peritoneal cell preparations (containing no detectable interferon or virus) produced significantly less interferon than cells from uninfected mice following exposure to Newcastle disease virus or Chikungunya virus. More importantly, hyporeactivity could be transferred to normal peritoneal cells incubated in serum (containing no detectable interferon or virus) from EMC virus-infected mice during the hyporeactive period. Incubation of peritoneal exudate cells with up to a 1:200 dilution of serum from hyporeactive animals still resulted in a significant reduction in the interferon response induced by Newcastle disease virus or Chikungunya virus. Furthermore, it could be shown that this serum hyporeactive factor was effective in mouse cells but not in rabbit, chicken, or human cells.
如先前报道，感染脑心肌炎（EMC）病毒的小鼠对干扰素诱导逐渐产生低反应状态，这表现为在感染开始96小时后给予四种不同诱导剂后，血清干扰素水平降低80%。在本报告中，在EMC病毒感染期间最大低反应性（96小时）时采集的腹膜渗出细胞中，这种低反应性在体外得到了进一步证实。这些腹膜细胞制剂（不含可检测到的干扰素或病毒）在接触新城疫病毒或基孔肯雅病毒后产生的干扰素明显少于未感染小鼠的细胞。更重要的是，低反应性可以转移到在低反应期来自EMC病毒感染小鼠的血清（不含可检测到的干扰素或病毒）中孵育的正常腹膜细胞。用低反应性动物血清稀释至1:200孵育腹膜渗出细胞，仍然会导致新城疫病毒或基孔肯雅病毒诱导的干扰素反应显著降低。此外，可以证明这种血清低反应性因子在小鼠细胞中有效，但在兔、鸡或人类细胞中无效。
