大鼠乳腺癌的体内和体外研究：肿瘤克隆系的建立
A rat mammary carcinoma in vivo and in vitro: establishment of clonal lines of the tumor.
作者信息
Kano-Sueoka T, Hsieh P
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1922-6. doi: 10.1073/pnas.70.7.1922.
Clonal cell lines from a pituitary hormone-dependent rat mammary carcinoma were established. The growth of these cell lines in vitro is markedly stimulated in the presence of prolactin. The cells grow well in a medium supplemented with fetal-calf serum (generation time: 9-10 hr), but do not grow with calf serum. Fetal-calf serum supports little growth when the serum is subjected to anti-prolactin-affinity chromatography. Gel filtration of fetal-calf serum indicates that the growth stimulatory activity is in a large molecular weight material (close to 100,000), suggesting that prolactin is associated with another serum component. A variant of the original tumor, which does not require hormone for growth, was obtained, and clonal cell lines of this tumor were also established. These cells, in contrast to the hormone-dependent ones, grow well with either fetal-calf serum (with or without anti-prolactin treatment) or calf serum.
建立了来自垂体激素依赖性大鼠乳腺癌的克隆细胞系。在催乳素存在的情况下，这些细胞系在体外的生长受到显著刺激。这些细胞在添加胎牛血清的培养基中生长良好（倍增时间：9 - 10小时），但在添加小牛血清的培养基中不生长。当胎牛血清经过抗催乳素亲和层析时，其支持的生长很少。对胎牛血清进行凝胶过滤表明，生长刺激活性存在于一种大分子物质中（接近100,000），这表明催乳素与另一种血清成分相关。获得了原始肿瘤的一个变体，其生长不需要激素，并建立了该肿瘤的克隆细胞系。与激素依赖性细胞系不同，这些细胞无论是在胎牛血清（有无抗催乳素处理）还是小牛血清中都生长良好。
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