Klein G, Steiner M, Wiener F, Klein E
Proc Natl Acad Sci U S A. 1974 Mar;71(3):685-9. doi: 10.1073/pnas.71.3.685.
A brilliant, coarsely granular nuclear antigen was detected by anti-complement immunofluorescence in the nuclei of acute myeloid leukemia myeloblasts. Designated as LANA (leukemia-associated nuclear antigen), the reactivity differs from that of the Epstein-Barr-virus-determined nuclear antigen (EBNA) in immunological specificity and morphological appearance, although it is visualized by the same method. Serum from acute myeloid leukemia patients gave positive reactions in 73% of the cases. In acute lymphatic leukemia, chronic myeloid leukemia, chronic lymphatic leukemia, and Burkitt's lymphoma the sera were positive in 35, 14, 19, and 24%, respectively. Two of five polycythemia and two of eleven myeloma sera were also positive. Among 61 healthy controls, 58 were negative, whereas three showed a diffuse nuclear staining with a different pattern. Among 24 carcinoma patients, 18 were negative, whereas six gave a nuclear staining with a different, diffuse pattern. Sera from 20 patients who had recovered from infectious mononucleosis were all negative. In addition to the blasts of acute myeloid leukemia, a similar reactivity was seen with two Epstein-Barr virus DNA and EBNA-negative African lymphoma biopsies and in a short-lived tissue culture line derived from one of them. LANA could be a fetal or tissue-specific antigen, a virally determined antigen, or a specific form of anti-nuclear reactivity.
通过抗补体免疫荧光法在急性髓系白血病原始粒细胞的细胞核中检测到一种明亮的、粗颗粒状核抗原。该抗原被命名为LANA（白血病相关核抗原），尽管其通过与爱泼斯坦-巴尔病毒确定的核抗原（EBNA）相同的方法显示，但在免疫特异性和形态外观上，其反应性与EBNA不同。急性髓系白血病患者的血清在73%的病例中呈阳性反应。在急性淋巴细胞白血病、慢性髓系白血病、慢性淋巴细胞白血病和伯基特淋巴瘤中，血清阳性率分别为35%、14%、19%和24%。五例真性红细胞增多症患者中的两例以及十一例骨髓瘤患者中的两例血清也呈阳性。在61名健康对照者中，58名呈阴性，而三名显示出不同模式的弥漫性核染色。在24名癌症患者中，18名呈阴性，而六名呈现出不同的、弥漫性模式的核染色。20名从传染性单核细胞增多症康复的患者的血清均为阴性。除急性髓系白血病的原始粒细胞外，在两份爱泼斯坦-巴尔病毒DNA和EBNA阴性的非洲淋巴瘤活检样本以及从其中一份样本衍生的短期组织培养系中也观察到类似的反应性。LANA可能是一种胎儿或组织特异性抗原、病毒决定的抗原或一种特定形式的抗核反应性。
