Easmon C S
Br J Exp Pathol. 1979 Feb;60(1):24-8.
Human blood phagocytes and opsonized Staphylococcus aureus were incubated together in vitro and then treated with the bacteriolytic enzyme lysostaphin in order to remove extracellular bacteria. After washing, the phagocytic cells were incubated for a further 90 min with or without added antibiotics and the effect of these drugs on the survival of sensitive intracellular Staph. aureus measured. Penicillin and cefazolin had no effect on intracellular staphylococcal survival, but rifampicin, streptomycin and gentamicin reduced intracellular bacterial counts. The intracellular survival of antibiotic-resistant strains of Staph. aureus was not affected by the relevant extracellular antibiotics. Pre-incubation of phagocytic cells with either aminoglycosides or rifampicin had no effect on the intracellular survival of antibiotic-sensitive Staph. aureus.
将人血吞噬细胞与经调理的金黄色葡萄球菌在体外共同孵育，然后用溶葡萄球菌酶进行处理以去除细胞外细菌。洗涤后，将吞噬细胞在添加或不添加抗生素的情况下再孵育90分钟，并测定这些药物对敏感细胞内金黄色葡萄球菌存活的影响。青霉素和头孢唑林对细胞内葡萄球菌的存活没有影响，但利福平、链霉素和庆大霉素可减少细胞内细菌数量。金黄色葡萄球菌耐药菌株在细胞内的存活不受相关细胞外抗生素的影响。用氨基糖苷类药物或利福平对吞噬细胞进行预孵育，对抗生素敏感的金黄色葡萄球菌在细胞内的存活没有影响。
