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用于初步鉴定肠球菌和链球菌的胆汁-七叶苷试验：胆汁浓度、接种技术和孵育时间的影响

Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time.

作者信息

Chuard C, Reller L B

机构信息

Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA.

出版信息

J Clin Microbiol. 1998 Apr;36(4):1135-6. doi: 10.1128/JCM.36.4.1135-1136.1998.

DOI:10.1128/JCM.36.4.1135-1136.1998
PMID:9542954
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC104706/
Abstract

The bile-esculin test is used to differentiate enterococci and group D streptococci from non-group D viridans group streptococci. The effects on test performance of the concentration of bile salts, inoculum, and duration of incubation were examined with 110 strains of enterococci, 30 strains of Streptococcus bovis, and 110 strains of non-group D viridans group streptococci. Optimal sensitivity (> 99%) and specificity (97%) of the bile-esculin test can be obtained with a bile concentration of 40%, a standardized inoculum of 10(6) CFU, and incubation for 24 h.

摘要

胆汁七叶苷试验用于区分肠球菌和D群链球菌与非D群草绿色链球菌。用110株肠球菌、30株牛链球菌和110株非D群草绿色链球菌检测了胆盐浓度、接种量和孵育时间对试验结果的影响。胆汁浓度为40%、标准化接种量为10(6)CFU、孵育24小时时，胆汁七叶苷试验可获得最佳敏感性（>99%）和特异性（97%）。

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本文引用的文献

1
The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).在肠球菌（D群链球菌）鉴定中使用胆汁七叶苷培养基及马克斯泰德氏兰斯菲尔德分组技术。
J Clin Pathol. 1954 May;7(2):160-3. doi: 10.1136/jcp.7.2.160.
2
An analysis of 110 serious enterococcal infections. Epidemiology, antibiotic susceptibility, and outcome.
Medicine (Baltimore). 1995 Jul;74(4):191-200. doi: 10.1097/00005792-199507000-00003.
3
Laboratory studies with a selective Enterococcus medium.使用选择性肠球菌培养基的实验室研究。
Appl Microbiol. 1970 Sep;20(3):433-6. doi: 10.1128/am.20.3.433-436.1970.
4
Comparison of selective media for isolation of presumptive group D streptococci from human feces.用于从人类粪便中分离推定的D群链球菌的选择性培养基的比较。
Appl Microbiol. 1971 Dec;22(6):1008-11. doi: 10.1128/am.22.6.1008-1011.1971.
5
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Appl Microbiol. 1970 Aug;20(2):245-50. doi: 10.1128/am.20.2.245-250.1970.
6
Evaluation of various media for isolation, enumeration and identification of fecal streptococci from natural sources.评估用于从自然来源分离、计数和鉴定粪链球菌的各种培养基。
Health Lab Sci. 1972 Oct;9(4):289-98.
7
Recognition of group D streptococcal species of human origin by biochemical and physiological tests.通过生化和生理学测试识别源自人类的D组链球菌菌种。
Appl Microbiol. 1972 Jun;23(6):1131-9. doi: 10.1128/am.23.6.1131-1139.1972.
8
Comparison of several laboratory media for presumptive identification of enterococci and group D streptococci.几种用于初步鉴定肠球菌和D群链球菌的实验室培养基的比较。
Appl Microbiol. 1973 Aug;26(2):138-45. doi: 10.1128/am.26.2.138-145.1973.
9
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Clin Microbiol Rev. 1990 Jan;3(1):46-65. doi: 10.1128/CMR.3.1.46.
10
Association of Streptococcus bovis with carcinoma of the colon.牛链球菌与结肠癌的关联。
N Engl J Med. 1977 Oct 13;297(15):800-2. doi: 10.1056/NEJM197710132971503.