用于初步鉴定肠球菌和链球菌的胆汁-七叶苷试验：胆汁浓度、接种技术和孵育时间的影响
Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time.
作者信息
Chuard C, Reller L B
机构信息
Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1135-6. doi: 10.1128/JCM.36.4.1135-1136.1998.
Abstract
The bile-esculin test is used to differentiate enterococci and group D streptococci from non-group D viridans group streptococci. The effects on test performance of the concentration of bile salts, inoculum, and duration of incubation were examined with 110 strains of enterococci, 30 strains of Streptococcus bovis, and 110 strains of non-group D viridans group streptococci. Optimal sensitivity (> 99%) and specificity (97%) of the bile-esculin test can be obtained with a bile concentration of 40%, a standardized inoculum of 10(6) CFU, and incubation for 24 h.
摘要
胆汁七叶苷试验用于区分肠球菌和D群链球菌与非D群草绿色链球菌。用110株肠球菌、30株牛链球菌和110株非D群草绿色链球菌检测了胆盐浓度、接种量和孵育时间对试验结果的影响。胆汁浓度为40%、标准化接种量为10(6)CFU、孵育24小时时，胆汁七叶苷试验可获得最佳敏感性（>99%）和特异性（97%）。
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The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).在肠球菌（D群链球菌）鉴定中使用胆汁七叶苷培养基及马克斯泰德氏兰斯菲尔德分组技术。
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