Király K, Jobbágy A, Mecher T
Bull World Health Organ. 1965;33(5):687-703.
Contradictory reports have been published on the value of fluorescent protein tracing as a serological test for syphilis. The authors believe that this is due mainly to differences in technique and especially to variations in the quality of the conjugate used. They describe the preparation of a fluorescein isothiocyanate (FITC) immune serum conjugate, which they characterize by its antihuman-globulin titre, FITC/protein ratio and staining effect. The fluorescent treponemal antibody (FTA-50) test performed with this conjugate gave better agreement with the TPI test than any of four other serological tests with which it was compared. Its sensitivity is also good, being equal to that of the T. pallidum complement-fixation (TPCF) test. Nevertheless, the FTA-50 test is not absolutely specific and, as a screening procedure before TPI testing, a combination of the TPCF test with the cardiolipin complement-fixation test appears to be simpler, more sensitive and cheaper. Further study is needed with a view to the elimination of non-specific staining and standardization of the conjugate and antigen before the FTA-50 test can replace the TPI test.
关于荧光蛋白追踪作为梅毒血清学检测方法的价值，已发表了相互矛盾的报告。作者认为，这主要是由于技术差异，尤其是所用结合物质量的差异。他们描述了异硫氰酸荧光素（FITC）免疫血清结合物的制备方法，并通过其抗人球蛋白滴度、FITC/蛋白质比率和染色效果对其进行了表征。用这种结合物进行的荧光密螺旋体抗体（FTA - 50）检测与TPI检测的一致性比与之比较的其他四种血清学检测中的任何一种都要好。其灵敏度也很高，与梅毒螺旋体补体结合（TPCF）检测相当。然而，FTA - 50检测并非绝对特异，作为TPI检测前的筛查程序，TPCF检测与心磷脂补体结合检测相结合似乎更简单、更灵敏且更便宜。在FTA - 50检测能够取代TPI检测之前，需要进一步研究以消除非特异性染色，并对结合物和抗原进行标准化。
