Davies A J, Carter R L, Leuchars E, Wallis V
Immunology. 1969 Jul;17(1):111-26.
The response to oxazolone, painted on the skin, has been studied in the regional lymph nodes of normal adult CBA/H mice and of thymectomized, X-irradiated, bone-marrow injected mice with or without a reconstituting thymus graft. The thymus grafts carry a marker chromosome so that, in a reconstituted animal, cells derived from bone marrow and from the thymus can be distinguished. The response to oxazolone appears to be biphasic and both thymus-derived and bone-marrow-derived cells participate in it. In the first stage, normal mice show a massive proliferation of thymus-derived cells in the paracortex which reaches a peak on day 4. This reaction is absent in thymectomized mice but is restored if animals are grafted with a syngeneic thymus. The second stage consists of proliferation of bone-marrow derived cells which is maximal on day 8 and is then sustained, at a slightly lower level, for the rest of the experiment. It coincides with germinal centre formation and hyperplasia of the medullary cords—changes which are best seen in normal and reconstituted mice though they are also present to a lesser extent in thymectomized animals. The part played by bone-marrow derived elements in the immune response to oxazolone is not known but that there is a considerable proliferation of these cells in the later stages in the present experimental model is certain.
已在正常成年CBA/H小鼠以及胸腺切除、X射线照射、注射骨髓且有或无重建胸腺移植的小鼠的局部淋巴结中，研究了涂抹于皮肤上的恶唑酮的反应。胸腺移植带有一条标记染色体，这样在重建动物中，可区分源自骨髓和胸腺的细胞。对恶唑酮的反应似乎是双相的，胸腺来源和骨髓来源的细胞都参与其中。在第一阶段，正常小鼠在副皮质区显示胸腺来源细胞的大量增殖，在第4天达到峰值。胸腺切除的小鼠没有这种反应，但如果给动物移植同基因胸腺，反应会恢复。第二阶段包括骨髓来源细胞的增殖，在第8天达到最大值，然后在实验的剩余时间里维持在略低水平。它与生发中心形成和髓索增生同时出现——这些变化在正常和重建小鼠中最明显，不过在胸腺切除的动物中也有程度较轻的表现。骨髓来源的细胞在对恶唑酮的免疫反应中所起的作用尚不清楚，但在本实验模型的后期这些细胞有相当程度的增殖是肯定的。
