Chiorazzi N, Eshhar Z, Katz D H
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2091-5. doi: 10.1073/pnas.73.6.2091.
High titered IgE, IgG, and IgM antibody responses to the major antigenic determinant of penicillin, the benzylpenicilloyl hapten, were elicited by the intraperitoneal injection of the hapten coupled to keyhole limpet hemocyanin mixed with the appropriate adjuvant. However, treatment of such mice with the benzylpenicilloyl derivatized synthetic copolymer of D-glutamic acid and D-lysine, either before or after primary immunization, resulted in significant suppression of the subsequent anti-benzylpenicilloyl antibody responses of the IgE and IgG classes, as measured at the humoral and cellular levels. The state of tolerance induced by benzylpenicili-poly(DGlu, Lys) was highly specific, of long duration, and could be induced in a manner that would be appropriate for clinical use. These results provide a direct demonstration of the potential application of the poly(DGlu, Lys) immunotherapeutic approach to penicillin allergy in humans.
通过腹腔注射与钥孔戚血蓝蛋白偶联的半抗原（并与适当佐剂混合），引发了针对青霉素主要抗原决定簇苄青霉素酰半抗原的高滴度IgE、IgG和IgM抗体反应。然而，在用苄青霉素酰衍生化的D-谷氨酸和D-赖氨酸合成共聚物处理此类小鼠时，无论是在初次免疫之前还是之后，均导致IgE和IgG类后续抗苄青霉素酰抗体反应在体液和细胞水平上受到显著抑制。苄青霉素-聚（D-谷氨酸，赖氨酸）诱导的耐受状态具有高度特异性、持续时间长，并且可以以适合临床应用的方式诱导产生。这些结果直接证明了聚（D-谷氨酸，赖氨酸）免疫治疗方法在人类青霉素过敏中的潜在应用。
