抗原特异性T淋巴细胞克隆。I. 表达抗原特异性抑制活性的T淋巴细胞克隆的特性
Antigen-specific T lymphocyte clones. I. Characterization of a T lymphocyte clone expressing antigen-specific suppressive activity.
作者信息
Fresno M, Nabel G, McVay-Boudreau L, Furthmayer H, Cantor H
出版信息
J Exp Med. 1981 May 1;153(5):1246-59. doi: 10.1084/jem.153.5.1246.
Abstract
We have generated continuously propagatable T lymphocyte clones to study antigen-specific T cell functions. All Ly-2+ clones mediate suppressive activity and secrete a characteristic pattern of polypeptides that differs from Ly-2- T cell clones. Cells of one clone, Cl.Ly23/4, specifically bind glycophorin from sheep erythrocytes (SRBC). After incubation with [35S]methionine, supernate material from this clone also contains biosynthetically labeled 70,000-mol wt proteins that specifically bind to SRBC and this binding is inhibited by glycophorin from sheep but not other erythrocytes. These antigen-binding 70,000-mol wt peptides specifically and completely suppress primary anti-SRBC responses generated by mixtures of primed Ly-1+2- cells and B cells. Suppression by these antigen-binding peptides reflects direct inhibition of T-helper activity.
摘要
我们已生成可连续传代的T淋巴细胞克隆，以研究抗原特异性T细胞功能。所有Ly-2+克隆均介导抑制活性，并分泌一种与Ly-2-T细胞克隆不同的特征性多肽模式。一个克隆Cl.Ly23/4的细胞可特异性结合绵羊红细胞（SRBC）的血型糖蛋白。用[35S]甲硫氨酸孵育后，该克隆的上清液中还含有经生物合成标记的70,000道尔顿分子量的蛋白质，这些蛋白质可特异性结合SRBC，且这种结合可被绵羊的血型糖蛋白抑制，但不能被其他红细胞的血型糖蛋白抑制。这些与抗原结合的70,000道尔顿分子量的肽可特异性且完全抑制由致敏的Ly-1+2-细胞和B细胞混合物产生的原发性抗SRBC反应。这些与抗原结合的肽的抑制作用反映了对T辅助活性的直接抑制。
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