D'Eustachio P, Ingram R S, Tilghman S M, Ruddle F H
Somatic Cell Genet. 1981 May;7(3):289-94. doi: 10.1007/BF01538854.
Several lines of evidence suggest a close functional relationship and a common evolutionary origin for alpha-fetoprotein and albumin. In the mouse, breeding studies have previously allowed the assignment of the albumin gene to chromosome 5. To test the possible linkage of alpha-fetoprotein and albumin, five somatic cell hybrids containing various combinations of mouse chromosomes, together with a constant set of hamster chromosomes, were tested for the presence of both genes using DNA restriction mapping techniques. Two of the five hybrids possessed both genes, and the other three lacked both. The only mouse chromosome present in the positive lines and absent from the negative ones was number 5, allowing the assignment of both genes to this chromosome.
几条证据线索表明甲胎蛋白和白蛋白之间存在密切的功能关系以及共同的进化起源。在小鼠中，先前的育种研究已将白蛋白基因定位到5号染色体。为了测试甲胎蛋白和白蛋白可能的连锁关系，利用DNA限制性图谱技术，对五个包含小鼠染色体不同组合以及一组恒定仓鼠染色体的体细胞杂种进行了这两种基因的检测。五个杂种中的两个同时拥有这两种基因，另外三个则都没有。阳性系中存在而阴性系中不存在的唯一小鼠染色体是5号染色体，这使得这两种基因都被定位到该染色体上。
