Carella G, Chatenoud L, Degos F, Bach M A
J Clin Immunol. 1982 Apr;2(2):93-100. doi: 10.1007/BF00916892.
Three monoclonal anti-T-cell antibodies, specifically directed against total T cells (OKT3), inducer-helper T cells (OKT4) and suppressor/cytotoxic T cells (OKT8), were used in this study to analyze peripheral T-cell subsets in hepatitis B surface antigen (HBsAg)-positive and -negative chronic active hepatitis (CAH) patients. Results showed that a clear-cut difference exists in the distribution of peripheral T cells of these two groups of subjects. HBsAg-positive CAH patients had a numerical predominance of peripheral T lymphocytes expressing the characteristics of cytotoxic/suppressor T cells. In contrast, patients with "autoimmune" HBsAg-negative CAH exhibit a predominance of OKT4 cells, namely, the helper-inducer T-cell subset. In addition, high numbers of circulating double labeled cells (expressing both the OKT4 and the OKT8 xenoantigens) were detected in some of the HBsAg-positive and HBsAg-negative CAH patients studied.
本研究使用了三种单克隆抗T细胞抗体，分别特异性针对总T细胞（OKT3）、诱导-辅助性T细胞（OKT4）和抑制/细胞毒性T细胞（OKT8），以分析乙肝表面抗原（HBsAg）阳性和阴性的慢性活动性肝炎（CAH）患者的外周血T细胞亚群。结果显示，这两组受试者外周血T细胞的分布存在明显差异。HBsAg阳性的CAH患者外周血中表达细胞毒性/抑制性T细胞特征的T淋巴细胞数量占优势。相反，“自身免疫性”HBsAg阴性的CAH患者表现为OKT4细胞占优势，即辅助-诱导性T细胞亚群。此外，在部分所研究的HBsAg阳性和HBsAg阴性的CAH患者中检测到大量循环双标记细胞（同时表达OKT4和OKT8异种抗原）。
