Liang T J, Isselbacher K J, Wands J R
Department of Medicine, Harvard Medical School, Massachusetts General Hospital Cancer Center, Charlestown 02129.
J Clin Invest. 1989 Oct;84(4):1367-71. doi: 10.1172/JCI114308.
A sensitive and specific method has been developed to detect hepatitis B virus (HBV) in serum. The method involves two steps: the capture of viral genome from serum using a high affinity IgM monoclonal antibody directed against a common a domain epitope found on the envelope, and the amplification of viral DNA by the polymerase chain reaction (PCR). The amplification is initiated using "generic" primers derived from the core and pre-core sequences which are highly conserved amongst the hepadnaviruses. This rapid technique detects less than 10 infectious virions and may be useful in the study of individuals with acute and chronic liver disease of unknown etiology.
已开发出一种灵敏且特异的方法来检测血清中的乙型肝炎病毒（HBV）。该方法包括两个步骤：使用针对包膜上常见α结构域表位的高亲和力IgM单克隆抗体从血清中捕获病毒基因组，以及通过聚合酶链反应（PCR）扩增病毒DNA。扩增使用源自核心和前核心序列的“通用”引物启动，这些序列在嗜肝DNA病毒中高度保守。这种快速技术可检测到少于10个感染性病毒粒子，可能有助于研究病因不明的急慢性肝病患者。
