Ruckdeschel J C, Mardiney M R
J Immunol Methods. 1976;13(2):131-43. doi: 10.1016/0022-1759(76)90151-4.
The demonstration of in vitro lymphocyte responsiveness to common pediatric viruses has previously been fraught with many technical and conceptual problems. Based upon our prior experience in demonstrating cell-associated immunity to mumps, rubella and measles viruses we illustrate our methodology and conceptual framework by documenting in vitro lymphocyte responsiveness to the Varicella-zoster virus, another ubiquitous virus of childhood. We discuss our approach to the problems of reactivity, specificity and reliability in the use of membrane-associated viral antigens.
此前，证明体外淋巴细胞对常见儿科病毒的反应性一直存在许多技术和概念上的问题。基于我们先前在证明对腮腺炎、风疹和麻疹病毒的细胞相关免疫方面的经验，我们通过记录体外淋巴细胞对水痘带状疱疹病毒（另一种儿童期常见病毒）的反应性，来说明我们的方法和概念框架。我们讨论了在使用膜相关病毒抗原时应对反应性、特异性和可靠性问题的方法。
