Crisp A J
Rheumatol Int. 1984;4(3):125-8. doi: 10.1007/BF00541181.
Fragments of synovial membranes from three patients with rheumatoid arthritis were incubated in Dulbecco's modified Eagle's medium (DMEM). Histamine was released into the medium at rates up to 649.3 ng/g synovium/day. To investigate whether histamine in synovial fluid may have more than a vasoactive role, cells cultured from human trabecular bone adjacent to a rheumatoid joint and human adherent rheumatoid synovial cells were incubated with different concentrations of histamine for 5 min. Histamine increased the intracellular content of cyclic AMP up to three fold in a dose-related manner but had no effect on synovial cells. This offers preliminary evidence that cells grown from human trabecular bone have histamine receptors.
将三名类风湿性关节炎患者的滑膜碎片置于杜氏改良 Eagle 培养基（DMEM）中培养。组胺以高达 649.3 纳克/克滑膜/天的速率释放到培养基中。为了研究滑液中的组胺是否可能具有不止血管活性作用，将来自类风湿关节附近的人小梁骨培养的细胞和人贴壁类风湿滑膜细胞与不同浓度的组胺一起孵育 5 分钟。组胺以剂量相关的方式使细胞内环磷酸腺苷（cAMP）的含量增加高达三倍，但对滑膜细胞没有影响。这提供了初步证据，表明从人小梁骨培养的细胞具有组胺受体。
