细菌视紫红质中席夫碱质子的交换动力学。
Exchange kinetics of the Schiff base proton in bacteriorhodopsin.
作者信息
Ehrenberg B, Lewis A, Porta T K, Nagle J F, Stoeckenius W
出版信息
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6571-3. doi: 10.1073/pnas.77.11.6571.
Abstract
Using rapid mixing techniques and resonance Raman spectroscopy, we have found that the 1H/2H exchange time for the Schiff base proton of bacteriorhodopsin in purple membrane is 4.7 msec, when experiments are carried out at pH 2 or pH 7 at room temperature in the dark. We argue that diffusion of neutral water into the membrane is fast on this time scale. Also, model Schiff bases in solution have a pKa between 6 and 7, and we show that such model Schiff bases have much faster exchange rates. Therefore, we conclude that the Schiff base proton in bacteriorhodopsin is protected from interaction with the medium, probably by interaction with a protein group, and this would account for a pKa considerably higher than 6-7.
摘要
利用快速混合技术和共振拉曼光谱，我们发现，在室温黑暗条件下于pH 2或pH 7进行实验时，紫膜中细菌视紫红质席夫碱质子的1H/2H交换时间为4.7毫秒。我们认为，在此时间尺度上中性水向膜内的扩散很快。此外，溶液中的模型席夫碱的pKa在6到7之间，并且我们表明此类模型席夫碱具有快得多的交换速率。因此，我们得出结论，细菌视紫红质中的席夫碱质子可能通过与蛋白质基团相互作用而免受与介质的相互作用影响，这将解释其pKa远高于6 - 7的原因。
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本文引用的文献
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