猿猴肉瘤病毒致癌基因类似物在人类细胞中的染色体定位。

Swan D C, McBride O W, Robbins K C, Keithley D A, Reddy E P, Aaronson S A

Proc Natl Acad Sci U S A. 1982 Aug;79(15):4691-5. doi: 10.1073/pnas.79.15.4691.

The primate cell-derived transforming gene (v-sis) of simian sarcoma virus (SSV) is represented as a single copy marker within cellular DNAs of mammalian species including human. The human analogue of v-sis can be distinguished from its rodent counterparts by Southern blotting analysis of EcoRI-restricted DNAs. By testing for the presence of the human v-sis-related fragment, c-sis (human), in somatic cell hybrids possessing varying numbers of human chromosome, as well as in segregants of such hybrids, it was possible to assign c-sis to human chromosome 22.

猿猴肉瘤病毒（SSV）的灵长类动物细胞衍生转化基因（v-sis）在包括人类在内的哺乳动物细胞DNA中表现为单拷贝标记。通过对经EcoRI酶切的DNA进行Southern印迹分析，可将人类v-sis的类似物与其啮齿动物对应物区分开来。通过检测具有不同数量人类染色体的体细胞杂种以及此类杂种的分离物中人类v-sis相关片段c-sis（人类）的存在情况，有可能将c-sis定位于人类22号染色体。