人类和小鼠B淋巴细胞及T淋巴细胞肿瘤的阶段特异性转化基因。

Stage-specific transforming genes of human and mouse B- and T-lymphocyte neoplasms.

作者信息

Lane M A, Sainten A, Cooper G M

出版信息

Cell. 1982 Apr;28(4):873-80. doi: 10.1016/0092-8674(82)90066-6.

Abstract

DNAs of 20 B- and T-lymphocyte neoplasms of human and mouse origin induced transformation of NIH/3T3 cells with high efficiencies, indicating that these neoplasms contained activated transforming genes that were detectable by transfection. Analysis of the susceptibility of the transforming activities of lymphocyte-neoplasm DNAs to digestion with restriction endonucleases indicated that the same or closely related transforming genes were activated in independent neoplasms representative of the same stage of normal cell differentiation. However, different transforming genes were activated in neoplasms representative of different stages of normal B- and T-lymphocyte differentiation. These results indicate that specific transforming genes are activated in neoplasms of discrete stages of differentiation within these cell lineages.

摘要

源自人和小鼠的20种B淋巴细胞和T淋巴细胞肿瘤的DNA高效诱导NIH/3T3细胞发生转化，这表明这些肿瘤含有可通过转染检测到的活化转化基因。对淋巴细胞肿瘤DNA转化活性对限制性内切酶消化的敏感性分析表明，在代表正常细胞分化相同阶段的独立肿瘤中，相同或密切相关的转化基因被激活。然而，在代表正常B淋巴细胞和T淋巴细胞分化不同阶段的肿瘤中，激活了不同的转化基因。这些结果表明，在这些细胞谱系中，特定的转化基因在离散分化阶段的肿瘤中被激活。