Lane M A, Sainten A, Cooper G M
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5185-9. doi: 10.1073/pnas.78.8.5185.
High molecular weight DNAs of five tumors induced by mouse mammary tumor virus (MMTV), two mouse mammary tumors induced by a chemical carcinogen, and one human mammary tumor cell line (MCF-7) were assayed for the presence of transmissible activated transforming genes by transfection of NIH 3T3 mouse cells. DNAs of all five MMTV-induced tumors, one chemical carcinogen-induced tumor, and the human tumor cell line induced transformation with high efficiencies (approximately 0.2 transformant per micrograms of DNA). NIH cells transformed by DNAs of MMTV-induced tumors did not contain exogenous MMTV DNA sequences, indicating that MMTV-induced mammary carcinomas contained activated cellular transforming genes that were not linked to viral DNA. The transforming activities of DNAs of all five MMTV-induced tumors, the chemical carcinogen-induced mouse tumor, and the human tumor cell line were inactivated by digestion with the restriction endonucleases Pvu II and Sac I, but not by BamHI, EcoRI, HindIII, Kpn I, or Xho I. These results indicate that the same or closely related transforming genes were activated in six different mouse mammary carcinomas, induced by either MMTV or a chemical carcinogen, and in a human mammary carcinoma cell line.
通过转染NIH 3T3小鼠细胞，检测了由小鼠乳腺肿瘤病毒（MMTV）诱导的5种肿瘤、由化学致癌物诱导的2种小鼠乳腺肿瘤以及1种人乳腺肿瘤细胞系（MCF - 7）的高分子量DNA中是否存在可传递的活化转化基因。所有5种MMTV诱导的肿瘤、1种化学致癌物诱导的肿瘤以及人肿瘤细胞系的DNA均能高效诱导转化（每微克DNA约产生0.2个转化体）。由MMTV诱导的肿瘤DNA转化的NIH细胞不含外源性MMTV DNA序列，这表明MMTV诱导的乳腺癌含有未与病毒DNA相连的活化细胞转化基因。所有5种MMTV诱导的肿瘤、化学致癌物诱导的小鼠肿瘤以及人肿瘤细胞系的DNA的转化活性在用限制性内切酶Pvu II和Sac I消化后失活，但用BamHI、EcoRI、HindIII、Kpn I或Xho I消化则不会。这些结果表明，在由MMTV或化学致癌物诱导的6种不同小鼠乳腺癌以及人乳腺癌细胞系中，相同或密切相关的转化基因被激活。
