Dalla-Favera R, Franchini G, Martinotti S, Wong-Staal F, Gallo R C, Croce C M
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4714-7. doi: 10.1073/pnas.79.15.4714.
Retroviral transforming genes, v-onc genes, are derived from normal cellular sequences that are called cellular onc (c-onc) genes. DNA from mouse-human somatic cell hybrids that have selectively lost human chromosomes was used in Southern blots to map the chromosomal location of two human onc genes. Cloned human homologues of retroviral onc genes were used as probes. Because the human c-fes gene, which is homologous to feline sarcoma virus, segregates concordantly with human chromosome 15, and the human c-myb gene, which is homologous to avian myeloblastosis virus onc genes, segregates concordantly with human chromosome 6, we have assigned the c-fes and the c-myb genes to human chromosomes 15 and 6, respectively. Nonrandom chromosomal defects involving these human chromosomes have been observed in neoplasms. These studies should be valuable in determining whether specific rearrangements involving these chromosomes result in the abnormal expression of these onc genes in human malignancies.
逆转录病毒转化基因，即v-onc基因，源自被称为细胞癌基因（c-onc）的正常细胞序列。利用选择性丢失人类染色体的小鼠-人类体细胞杂种的DNA进行Southern印迹分析，以确定两个人类癌基因的染色体定位。克隆的逆转录病毒癌基因的人类同源物用作探针。由于与猫肉瘤病毒同源的人类c-fes基因与人类15号染色体协同分离，而与禽成髓细胞瘤病毒癌基因同源的人类c-myb基因与人类6号染色体协同分离，因此我们分别将c-fes和c-myb基因定位到人类15号和6号染色体上。在肿瘤中已观察到涉及这些人类染色体的非随机染色体缺陷。这些研究对于确定涉及这些染色体的特定重排是否会导致这些癌基因在人类恶性肿瘤中的异常表达具有重要价值。
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