McCance D J, Walker P G, Dyson J L, Coleman D V, Singer A
Br Med J (Clin Res Ed). 1983 Sep 17;287(6395):784-8. doi: 10.1136/bmj.287.6395.784.
Twenty two patients referred to a district colposcopy clinic because of an abnormal cervical cytology report or a suspicious cervix and found to have a cervical epithelial abnormality were studied. The techniques of cytology, histology, immunohistochemistry, and DNA-DNA hybridisation were used to detect infection by human papillomavirus. Using an indirect immunoalkaline phosphatase technique human papillomavirus antigen was found in biopsy specimens from six of the 22 patients and DNA of papillomavirus type 6 in biopsy specimens from 13 of these women, including four out of six whose histological diagnosis was cervical intraepithelial neoplasia grade 3. In eight cases where cytological, colposcopical, and histological investigations all indicated the presence of wart virus infection, papillomavirus type 6 DNA was found in seven. Papillomavirus type 6 DNA was found in more than half of the proved cases of cervical intraepithelial neoplasia. The presence of this viral DNA in women with no cervical abnormality is to be studied.
对22名因宫颈细胞学检查报告异常或宫颈可疑而转诊至地区阴道镜诊所且被发现有宫颈上皮异常的患者进行了研究。采用细胞学、组织学、免疫组织化学和DNA-DNA杂交技术检测人乳头瘤病毒感染。使用间接免疫碱性磷酸酶技术，在22名患者中的6名患者的活检标本中发现了人乳头瘤病毒抗原，在其中13名女性的活检标本中发现了6型乳头瘤病毒的DNA，包括6名组织学诊断为宫颈上皮内瘤变3级患者中的4名。在8例细胞学、阴道镜检查和组织学检查均表明存在疣病毒感染的病例中，7例发现了6型乳头瘤病毒DNA。在超过一半的经证实的宫颈上皮内瘤变病例中发现了6型乳头瘤病毒DNA。无宫颈异常女性中这种病毒DNA的存在情况有待研究。
