小鼠中针对聚（ADP - 核糖）的抗体及抗体产生的比较研究。
Comparative studies on antibody and antibody production to poly(ADP-ribose) in mice.
作者信息
Kanai Y, Miwa M, Matsushima T, Sugimura T
出版信息
Immunology. 1978 Mar;34(3):501-8.
Antibodies to poly(ADP-ribose) were produced in C3H/He mice by injection of poly(ADP-ribose) in Freund's incomplete adjuvant or its complexes with methylated bovine serum albumin in Freund's incomplete adjuvant. Titres of the antibody obtained from the latter were about 50-fold higher than those from the former. Thus, the effect of methylated bovine serum albumin on the antibody production to poly(ADP-ribose) resembled the case of poly(I).poly(C) and was different from the case of single stranded DNA. The class of the antibodies obtained from these two different procedures mainly consists of 7S antibodies, and the specificity of these antibodies was independent of their titres. It was also found that antibodies to poly(ADP-ribose) were most reactive to poly(ADP-ribose) with 20 repeating ADP-ribose units. The reactivity of the antibody was dependent on the chain length of the polymer. Neither the complex in Freund's incomplete adjuvant nor poly(ADP-ribose) alone in Freund's imcomplete adjuvant could induce an immune response to poly(ADP-ribose) in athymic nude mice. Therefore, the immune response to poly(ADP-ribose) may occur through a thymus function.
通过在弗氏不完全佐剂中注射聚（ADP - 核糖）或其与甲基化牛血清白蛋白在弗氏不完全佐剂中的复合物，在C3H/He小鼠中产生了抗聚（ADP - 核糖）抗体。从后者获得的抗体效价比前者高约50倍。因此，甲基化牛血清白蛋白对聚（ADP - 核糖）抗体产生的影响类似于聚（I）·聚（C）的情况，与单链DNA的情况不同。从这两种不同方法获得的抗体类别主要由7S抗体组成，并且这些抗体的特异性与其效价无关。还发现抗聚（ADP - 核糖）抗体对具有20个重复ADP - 核糖单元的聚（ADP - 核糖）反应最强。抗体的反应性取决于聚合物的链长。弗氏不完全佐剂中的复合物或弗氏不完全佐剂中的聚（ADP - 核糖）单独都不能在无胸腺裸鼠中诱导对聚（ADP - 核糖）的免疫反应。因此，对聚（ADP - 核糖）的免疫反应可能通过胸腺功能发生。
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