Sahm D F, Baker C N, Jones R N, Thornsberry C
J Clin Microbiol. 1984 Sep;20(3):561-7. doi: 10.1128/jcm.20.3.561-567.1984.
The influence of culture medium of the MICs of eight cephalosporins for 45 strains of Streptococcus faecalis was investigated. The MICs of cephalothin, cefamandole, and cefoperazone were not substantially influenced by the type of culture medium used. In contrast, MICs of cefuroxime, ceftizoxime, cefotaxime, cefmenoxime, and ceftriaxone varied markedly with both the commercial brand and the blood content of the broth used. The use of Mueller-Hinton broths (from Oxoid Ltd., GIBCO Diagnostics, and Difco Laboratories) supplemented with 5% lysed sheep blood frequently resulted in MICs that were greater than or equal to 16 times lower than the MICs obtained with these same broths without blood. Similar, but less marked, patterns were observed when supplemented and unsupplemented brain heart infusion and Sceptor broths were used. The influence of the broth on MICs suggests a complex interaction between some cephalosporins, medium components, and organisms. The cephalosporins that were affected by media share an identical moiety at the 7-acyl position (cefuroxime is slightly different), but this structure is not shared by those cephalosporins that were not affected. This commonality in structure at the 7-acyl position may be partially responsible for the observed results.
研究了培养基对45株粪肠球菌的8种头孢菌素最低抑菌浓度（MIC）的影响。头孢噻吩、头孢孟多和头孢哌酮的MIC受所用培养基类型的影响不大。相比之下，头孢呋辛、头孢唑肟、头孢噻肟、头孢甲肟和头孢曲松的MIC随所用肉汤的商业品牌和血液含量的不同而有显著差异。使用添加5%溶解羊血的Mueller-Hinton肉汤（来自Oxoid有限公司、GIBCO诊断公司和Difco实验室），其MIC往往比未添加血液的相同肉汤所获得的MIC低16倍以上。当使用添加和未添加血液的脑心浸液和Sceptor肉汤时，观察到类似但不太明显的模式。肉汤对MIC的影响表明，一些头孢菌素、培养基成分和生物体之间存在复杂的相互作用。受培养基影响的头孢菌素在7-酰基位置有相同的部分（头孢呋辛略有不同），但未受影响的头孢菌素不具有这种结构。7-酰基位置结构的这种共性可能是观察到这些结果的部分原因。
