Nouri-Aria K T, Hegarty J E, Alexander G J, Eddleston A L, Williams R
Clin Exp Immunol. 1985 Aug;61(2):290-6.
In vitro IgG production was measured using peripheral blood mononuclear cells from patients with autoimmune chronic active hepatitis (CAH) to determine whether the increased serum IgG levels were related to abnormalities of T or B lymphocyte function. A marked increase in spontaneous and pokeweed mitogen-induced proliferation of IgG producing cells was observed in 30 patients with untreated autoimmune CAH when compared with 25 normal subjects and 21 patients with autoimmune disease in whom a remission had been induced and maintained by prednisolone (P less than 0.01). Co-culture experiments clearly demonstrated that abnormalities of T lymphocyte function in untreated autoimmune CAH were responsible for the heightened IgG production in vitro. Pre-incubation of T lymphocytes from untreated patients with 5 X 10(-8)M prednisolone significantly reduced the number of cells producing IgG (P less than 0.05), suggesting that the modulation of the immune response following corticosteroid therapy is likely to be due to an alteration in T lymphocyte function.
使用自身免疫性慢性活动性肝炎（CAH）患者的外周血单个核细胞来测定体外IgG产生情况，以确定血清IgG水平升高是否与T或B淋巴细胞功能异常有关。与25名正常受试者和21名已通过泼尼松龙诱导并维持缓解的自身免疫性疾病患者相比，在30名未经治疗的自身免疫性CAH患者中观察到产生IgG的细胞自发增殖和商陆有丝分裂原诱导的增殖显著增加（P<0.01）。共培养实验清楚地表明，未经治疗的自身免疫性CAH中T淋巴细胞功能异常是体外IgG产生增加的原因。用5×10(-8)M泼尼松龙对未经治疗患者的T淋巴细胞进行预孵育可显著减少产生IgG的细胞数量（P<0.05），这表明皮质类固醇治疗后免疫反应的调节可能是由于T淋巴细胞功能的改变。
