Easmon C S, Crane J P
Br J Exp Pathol. 1984 Dec;65(6):725-30.
Neither clindamycin nor lincomycin killed intracellular Staphylococcus aureus over a 4-h period. Bio-assay of neutrophil sonicates after exposure to antibiotic showed the presence of active clindamycin at approximately 20 times the extracellular concentration. Clindamycin and lincomycin kill staphylococci relatively slowly, particularly in cell culture medium and balanced salt solution. This might account for their failure to kill intracellular staphylococci despite intracellular accumulation. The neutrophil experiments could not be extended to a time period (20 h) over which the antibiotics would kill S. aureus, as the presence of these bacteria within neutrophils for this length of time resulted in considerable cell lysis.
在4小时内，克林霉素和林可霉素均未杀死细胞内的金黄色葡萄球菌。对抗生素暴露后的中性粒细胞超声裂解物进行生物测定，结果显示活性克林霉素的存在量约为细胞外浓度的20倍。克林霉素和林可霉素杀死葡萄球菌的速度相对较慢，尤其是在细胞培养基和平衡盐溶液中。这可能解释了尽管它们在细胞内蓄积，但仍无法杀死细胞内葡萄球菌的原因。中性粒细胞实验无法延长至抗生素能够杀死金黄色葡萄球菌的时间段（20小时），因为中性粒细胞内存在这些细菌达此时长会导致大量细胞裂解。
