Shimada T, Sakazaki R, Horigome K, Uesaka Y, Niwano K
Jpn J Med Sci Biol. 1984 Jun;37(3):141-4. doi: 10.7883/yoken1952.37.141.
A total of 249 strains of mesophilic Aeromonas including 179 A. hydrophila and 70 A. caviae were tested for production of cholera-like enterotoxin by reversed passive latex agglutination (RPLA) assay. A cholera-like enterotoxin neutralized with cholera antitoxin was demonstrated in the culture filtrates from eight (4.5%) of the 179 A. hydrophila strains, while none of A. caviae strains revealed the enterotoxin production in the test. Production of the cholera-like enterotoxin in the eight strains of A. hydrophila was also confirmed by enzyme-linked immunosorbent assay (ELISA).
总共对249株嗜温气单胞菌进行了检测,其中包括179株嗜水气单胞菌和70株豚鼠气单胞菌,采用反向被动乳胶凝集试验(RPLA)检测霍乱样肠毒素的产生情况。在179株嗜水气单胞菌菌株中的8株(4.5%)的培养滤液中,证实存在用霍乱抗毒素中和的霍乱样肠毒素,而在检测中,豚鼠气单胞菌菌株均未显示出肠毒素产生。通过酶联免疫吸附测定(ELISA)也证实了8株嗜水气单胞菌中霍乱样肠毒素的产生。