Toothaker R D, Elmer G W
Antimicrob Agents Chemother. 1984 Oct;26(4):552-6. doi: 10.1128/AAC.26.4.552.
Saccharomyces boulardii, a yeast used in a number of countries for general and antibiotic-associated gastrointestinal illnesses, was examined for possible application in the prevention of clindamycin-induced mortality in the hamster colitis model. Hamsters were given free access to an aqueous 5% suspension of lyophilized yeast for 3 days before and 10 days after administration of a single oral clindamycin dose of from 0.2 to 0.8 mg/kg. Mortality was recorded in groups of 7 to 20 animals every 24 h for 10 to 30 days. Mean cecal concentrations of S. boulardii were greater than 10(6) CFU/ml throughout the yeast administration period. Yeast treatment significantly decreased cumulative percent mortality by an average of 29%. Death onset was not affected by yeast treatment. Cecitis was present in 86% of moribund animals (N = 95) and was absent in all surviving animals examined (N = 27). Toxigenic Clostridium difficile was isolated from 13 of 14 moribund hamsters examined. No adverse effects of the yeast treatment were observed in animals receiving S. boulardii without clindamycin. The results suggest that S. boulardii warrants further evaluation for the prevention of antibiotic-associated colitis.
布拉酵母菌在许多国家被用于治疗普通胃肠道疾病及抗生素相关性胃肠道疾病，本研究考察了其在仓鼠结肠炎模型中预防克林霉素诱导的死亡的潜在应用价值。在单次口服剂量为0.2至0.8mg/kg克林霉素之前3天及之后10天，给予仓鼠自由饮用冻干酵母的5%水悬浮液。每24小时记录7至20只动物一组的死亡率，持续10至30天。在整个酵母给药期间，盲肠中布拉酵母菌的平均浓度均大于10(6) CFU/ml。酵母治疗使累积死亡率显著降低，平均降低了29%。酵母治疗对死亡起始时间没有影响。86%的濒死动物（n = 95）存在盲肠炎，而所有接受检查的存活动物（n = 27）均未出现盲肠炎。在14只接受检查的濒死仓鼠中，有13只分离出产毒艰难梭菌。在未接受克林霉素的动物中给予布拉酵母菌，未观察到酵母治疗的不良反应。结果表明，布拉酵母菌在预防抗生素相关性结肠炎方面值得进一步评估。
