DNA片段固相磷酸三酯合成的质谱监测
Mass spectrometric monitoring of solid phase phosphate triester synthesis of DNA fragments.
作者信息
Alder L, Rosenthal A, Cech D
出版信息
Nucleic Acids Res. 1983 Dec 10;11(23):8431-41. doi: 10.1093/nar/11.23.8431.
Abstract
A new and convenient pyrolysis mass spectrometric approach for monitoring solid phase phosphate triester synthesis of oligodeoxyribonucleotides in 5'-3' direction has been developed. The base-typical fragment ions produced by pyrolysis at 280 degrees C of the polymer-bound oligonucleotide triesters in the mass spectrometer permit the analytical monitoring of DNA chain growth, using simple mathematical operations. The base composition can be determined directly from the polymer. In addition, minor nucleosides can easily be detected.
摘要
一种新型便捷的热解质谱方法已被开发出来，用于监测5'-3'方向寡脱氧核糖核苷酸固相磷酸三酯的合成。在质谱仪中，聚合物结合的寡核苷酸三酯在280℃热解产生的碱基典型碎片离子，通过简单的数学运算即可实现对DNA链生长的分析监测。碱基组成可直接从聚合物中确定。此外，少量核苷也能轻松检测到。
相似文献
1
Mass spectrometric monitoring of solid phase phosphate triester synthesis of DNA fragments.DNA片段固相磷酸三酯合成的质谱监测
Nucleic Acids Res. 1983 Dec 10;11(23):8431-41. doi: 10.1093/nar/11.23.8431.
2
A rapid deprotection procedure for phosphotriester DNA synthesis.一种用于磷酸三酯DNA合成的快速脱保护程序。
Nucleic Acids Res. 1984 Sep 11;12(17):6853-9. doi: 10.1093/nar/12.17.6853.
3
Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.聚合物载体寡核苷酸合成 XVIII：脱氧核苷的β-氰基乙基-N，N-二烷基氨基-/N-吗啉代亚磷酰胺用于 DNA 片段合成，简化最终产物的脱保护和分离
Nucleic Acids Res. 1984 Jun 11;12(11):4539-57. doi: 10.1093/nar/12.11.4539.
4
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.通过磷酸三酯中间体合成寡核苷酸的新有效方法。
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675.
5
Solid-phase synthesis of polynucleotides. VIII: A simplified synthesis of oligodeoxyribonucleotides.多核苷酸的固相合成。VIII：寡脱氧核糖核苷酸的简化合成。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:383-91. doi: 10.1101/sqb.1983.047.01.045.
6
Solid phase synthesis of oligonucleotides on a crosslinked polyacrylmorpholide support.在交联聚丙烯酰吗啉载体上进行寡核苷酸的固相合成。
Nucleic Acids Symp Ser. 1980(7):233-41.
7
8
Synthesis and conformational analysis of synthetic DNA fragments.合成DNA片段的合成与构象分析。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:403-9. doi: 10.1101/sqb.1983.047.01.047.
9
Development in the phosphite-triester method of synthesis of oligonucleotides.亚磷酸三酯法合成寡核苷酸的进展。
Nucleic Acids Symp Ser. 1980(7):133-45.
本文引用的文献
1
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.通过磷酸三酯中间体合成寡核苷酸的新有效方法。
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675.
2
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.寡脱氧核糖核苷酸的快速合成。VII. 在硅藻土-聚酰胺载体上通过连续流动磷酸三酯法固相合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243.
3
Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.脱氧寡核苷酸固相磷三酯合成法的改进
Nucleic Acids Res. 1982 Sep 25;10(18):5605-20. doi: 10.1093/nar/10.18.5605.
4
Syringe method for stepwise chemical synthesis of oligonucleotides.用于寡核苷酸逐步化学合成的注射器法
Nucleic Acids Res. 1982 May 25;10(10):3249-60. doi: 10.1093/nar/10.10.3249.
5
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.多核苷酸的固相合成。VI. 关于用于固相载体的聚苯乙烯共聚物的进一步研究。
Nucleic Acids Res. 1982 Mar 11;10(5):1755-69. doi: 10.1093/nar/10.5.1755.
6
Color coded triarylmethyl protecting groups useful for deoxypolynucleotide synthesis.用于脱氧多核苷酸合成的颜色编码三芳基甲基保护基团。
Nucleic Acids Res. 1983 Mar 11;11(5):1589-99. doi: 10.1093/nar/11.5.1589.
7
Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.硅胶：用于寡核苷酸固相磷酸三酯合成的一种改进载体。
Nucleic Acids Res. 1982 Nov 25;10(22):7439-48. doi: 10.1093/nar/10.22.7439.
8
Synthesis of oligodeoxyribonucleotides on silica gel support.硅胶载体上寡脱氧核糖核苷酸的合成。
Nucleic Acids Res. 1981 Jun 25;9(12):2807-17. doi: 10.1093/nar/9.12.2807.
9
Automated synthesis of gene fragments.基因片段的自动化合成
Science. 1981 Oct 16;214(4518):270-4. doi: 10.1126/science.6169150.
10
Sequence analysis of oligodeoxyribonucleotides by mass spectrometry. 1. Dinucleoside monophosphates.通过质谱法对寡脱氧核糖核苷酸进行序列分析。1. 二磷酸核苷单酯。
Biochemistry. 1977 Mar 22;16(6):1044. doi: 10.1021/bi00625a003.
Zotero 插件