急性早幼粒细胞白血病（APL）中15q+;17q-染色体易位的发生率。

Incidence of the 15q+;17q- chromosome translocation in acute promyelocytic leukaemia (APL).

作者信息

Sheer D, Lister T A, Amess J, Solomon E

出版信息

Br J Cancer. 1985 Jul;52(1):55-8. doi: 10.1038/bjc.1985.148.

DOI:10.1038/bjc.1985.148

PMID:3860247

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1977183/

Abstract

Cytogenetic analysis was carried out on peripheral blood cultures from seven patients with acute promyelocytic leukaemia (APL-M3). A reciprocal 15;17 chromosome translocation, t(15q+;17q-), was found in all cases, and the breakpoints estimated to be 15q22 and 17q12-21. In addition to the t(15q+;17q-), trisomy 10 was found in 50% of cells analysed in one case. These results suggest that the 15;17 chromosome translocation may be observed in most cases of APL where the leukaemic cells are cultured before cytogenetic analysis is performed. The use of conditioned media in the culture of leukaemic cells is also described.

摘要

对7例急性早幼粒细胞白血病（APL - M3）患者的外周血培养物进行了细胞遗传学分析。所有病例均发现15号和17号染色体相互易位，即t(15q+;17q-)，断点估计在15q22和17q12 - 21。除了t(15q+;17q-)，在1例患者50%分析的细胞中发现了10号染色体三体。这些结果表明，在大多数APL病例中，若在进行细胞遗传学分析前对白血病细胞进行培养，可能会观察到15号和17号染色体易位。还描述了白血病细胞培养中条件培养基的使用。