Samaranayake L P, MacFarlane T W
Arch Oral Biol. 1982;27(10):869-73. doi: 10.1016/0003-9969(82)90043-7.
An in-vitro technique was used to investigate the effects of oral commensal bacteria, serum, saliva, germ-tube formation and pH on the adherence of two strains of C. albicans to HeLa cells. Streptococcus salivarius and Streptococcus mitior reduced candidal adhesion whereas Streptococcus mutans had no significant effect. A mixed salivary pellicle on HeLa cells significantly enhanced candidal adhesion and a serum layer had no effect. Yeasts pre-incubated in whole saliva for 3 h showed significantly greater adhesion to HeLa and human embryonic kidney epithelial cells than yeasts in phosphate-buffered saline. The adherence of hyphal phase candida was significantly greater than in the blastospore phase. Adhesion varied with the pH of the test medium, maximal adherence was at pH 3 with less under neutral pH conditions. The factors involved in the adhesion of C. albicans to epithelial surfaces are complex and may play a rôle in the aetiopathology of human mucosal candidoses.
采用体外技术研究口腔共生菌、血清、唾液、芽管形成和pH值对两株白色念珠菌黏附于HeLa细胞的影响。唾液链球菌和缓症链球菌可降低念珠菌的黏附，而变形链球菌无显著影响。HeLa细胞上的混合唾液薄膜显著增强念珠菌的黏附，血清层则无影响。在全唾液中预孵育3小时的酵母对HeLa细胞和人胚肾上皮细胞的黏附显著高于在磷酸盐缓冲盐水中的酵母。菌丝相念珠菌的黏附显著高于芽生孢子相。黏附随测试培养基的pH值而变化，最大黏附发生在pH 3时，中性pH条件下较少。白色念珠菌黏附于上皮表面所涉及的因素很复杂，可能在人类黏膜念珠菌病的病因病理学中起作用。
