Aldershvile J, Caspani B, Frösner G G
J Clin Microbiol. 1980 May;11(5):458-61. doi: 10.1128/jcm.11.5.458-461.1980.
A radioimmunological neutralization assay for the determination of hepatitis B e antibody is described. The method is compared with a blocking and a competitive assay and with immunodiffusion. The neutralization assay proved to be the most sensitive method, and results obtained by this method were reproduced most easily. The frequency of nonspecific results may be up to 1% of the sera tested. Among patients with acute type B hepatitis, e antibody was found regularly soon after the clearance of hepatitis B e antigen by using the neutralization assay, whereas this antibody was found on the average several weeks later and in a lower percentage of patients by using the other methods. It is concluded that at present the neutralization assay is the method of choice.
本文描述了一种用于测定乙型肝炎e抗体的放射免疫中和试验。该方法与阻断试验、竞争试验以及免疫扩散法进行了比较。结果表明，中和试验是最灵敏的方法，且用该方法获得的结果最易于重复。非特异性结果的出现频率可能高达所检测血清的1%。在急性乙型肝炎患者中，使用中和试验时，在乙型肝炎e抗原清除后不久就能经常检测到e抗体，而使用其他方法时，平均要在几周后才能检测到该抗体，且检测到的患者比例较低。结论是，目前中和试验是首选方法。
