Morgan E L, Weigle W O
J Exp Med. 1980 Jan 1;151(1):1-11. doi: 10.1084/jem.151.1.1.
Murine splenic lymphocytes proliferate in response to supernatant material derived from Fc fragment-pulsed splenic adherent cells. The stimulatory supernatant results from the interaction of Fc fragments with adherent cells or adherent cell supernate. Isolation of the stimulatory material in the supernate by Sephadex chromatography revealed that the mitogenic component was a cleavage product of Fc with a mol wt of approximately 14,000. The spleen cell type responsible for the generation of mitogenic Fc subfragments appears to be a macrophage. Unstimulated macrophages release an active supernate without being exposed to Fc fragments. The supernate of unstimulated macrophages apparently contain an enzyme which is capable of cleaving Fc fragments into the 14,000-mol wt mitogenic molecules. The spleen cell population induced to proliferate in response to the adherent cell supernate is present in T-cell depleted and Sephadex G-10 filtered cell preparations. Depletion of cells bearing immunoglobulin on their surfaces results in a reduced proliferative response to the mitogenic supernatant material indicating that it is probably a B cell.
小鼠脾淋巴细胞对来自Fc片段脉冲处理的脾黏附细胞的上清液物质产生增殖反应。刺激上清液是Fc片段与黏附细胞或黏附细胞上清液相互作用的结果。通过葡聚糖凝胶色谱法分离上清液中的刺激物质，发现促有丝分裂成分是一种分子量约为14,000的Fc裂解产物。负责产生促有丝分裂Fc亚片段的脾细胞类型似乎是巨噬细胞。未刺激的巨噬细胞在未接触Fc片段的情况下释放出活性上清液。未刺激的巨噬细胞的上清液显然含有一种能够将Fc片段切割成分子量为14,000的促有丝分裂分子的酶。在T细胞耗竭和经葡聚糖凝胶G-10过滤的细胞制剂中，存在因黏附细胞上清液而被诱导增殖的脾细胞群体。表面带有免疫球蛋白的细胞的耗竭导致对促有丝分裂上清液物质的增殖反应降低，表明其可能是B细胞。
