B淋巴细胞被IgG的Fc区域激活。
B-lymphocytes activation by the Fc region of IgG.
作者信息
Berman M A, Weigle W O
出版信息
J Exp Med. 1977 Jul 1;146(1):241-56. doi: 10.1084/jem.146.1.241.
Strong stimulation of DNA synthesis (up to 150-fold) and blast transformation can be induced in mouse spleen cells by Fc fragments of human IgG. The mitogenic response is optimal on day 5 of culture and is dependent on the concentration of Fc fragments with a sedimentation rate of 3-5S. Intact IgG is also stimulatory, but only when modified by heat aggregation, and produces only a 10-fold increase in [3H]thymidine uptake. The stimulation by aggregated IgG is dependent on the Fc portion, since aggregated (or soluble) Fab or F(ab')2 fragments are inactive. The results show that the response is T-cell independent and that it is a function of nylon wool adherent, surface Ig-positive, Fc receptor-bearing B lymphocytes. Fc fragments do not induce plaque-forming cells to human IgG in normal mouse spleen cell cultures, but rather trigger polyclonal antibody synthesis (anti-goat erythrocytes, anti-2,4,6-trinitrophenyl). It is postulated that the Fc region of antibodies plays a role in the regulation of the humoral immune response by triggering clonal expansion of B lymphocytes.
人IgG的Fc片段可在小鼠脾细胞中诱导强烈的DNA合成刺激（高达150倍）和母细胞转化。促有丝分裂反应在培养第5天最佳，且取决于沉降率为3 - 5S的Fc片段浓度。完整的IgG也具有刺激作用，但仅在经热聚集修饰时才有，且仅使[³H]胸苷摄取增加10倍。聚集IgG的刺激作用取决于Fc部分，因为聚集的（或可溶性的）Fab或F(ab')₂片段无活性。结果表明，该反应不依赖T细胞，是尼龙毛黏附的、表面Ig阳性、带有Fc受体的B淋巴细胞的功能。在正常小鼠脾细胞培养中，Fc片段不会诱导针对人IgG的噬斑形成细胞，而是触发多克隆抗体合成（抗山羊红细胞、抗2,4,6 - 三硝基苯）。据推测，抗体的Fc区域通过触发B淋巴细胞的克隆扩增在体液免疫反应的调节中发挥作用。
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