Lu S H, Camus A M, Tomatis L, Bartsch H
J Natl Cancer Inst. 1981 Jan;66(1):33-6.
Extracts of pickled vegetables commonly consumed in Linhsien County, a high-incidence area for esophageal cancer in Northern China, were studied for mutagenicity. The liquid residue from ethereal extracts produced a dose-dependent increase of mutants in Salmonella typhimurium TA98 and TA100 strains; mutagenicity required the presence of a fortified liver microsomal activation system induced by Aroclor 1254 in adult male BD VI inbred rats. An amount of extract equivalent to 2.8 g fresh pickled vegetables produced sixfold (75 revertants/g) and twofold (45 revertants/g) increases in revertant frequencies in strains TA98 and TA100, respectively. Roussin's red methyl ester, a tetranitroso compound, [(NO)2Fe(CH3S)]2, not previously reported to occur in nature, was isolated and identified from the ethereal extracts. The synthetic compound was mutagenic in strain TA100 in the presence of a liver activation system, producing 25 revertants/mumol. Findings on the presence of mutagenic compounds in pickled vegetables were discussed in relation to their possible etiologic role in cancer of the esophagus in Linhsien County.
对中国北方食管癌高发区林县常见的腌制蔬菜提取物进行了致突变性研究。乙醚提取物的液体残渣在鼠伤寒沙门氏菌TA98和TA100菌株中产生了剂量依赖性的突变体增加；致突变性需要在成年雄性BD VI近交系大鼠中由Aroclor 1254诱导的强化肝微粒体激活系统的存在。相当于2.8克新鲜腌制蔬菜的提取物量分别使TA98和TA100菌株的回复突变频率增加了六倍（75个回复突变体/克）和两倍（45个回复突变体/克）。从乙醚提取物中分离并鉴定出了 Roussin 红甲酯这种四亚硝基化合物[(NO)2Fe(CH3S)]2，此前未曾报道其在自然界中存在。该合成化合物在肝激活系统存在的情况下对TA100菌株具有致突变性，产生25个回复突变体/微摩尔。讨论了腌制蔬菜中致突变化合物的存在情况与其在林县食管癌可能的病因学作用之间的关系。
