Muchmore H G, Scott E N, Felton F G, Fromtling R A
Mycopathologia. 1982 Apr 23;78(1):41-5. doi: 10.1007/BF00436580.
Clearance of cryptococcal polysaccharide (CP) from tissues and body fluids of nonimmune mice was studied. Mice were injected intravenously only with one mg of purified CP, and serum, urine and tissues were obtained from each animal at various intervals for a period of 84 days. Tissue extracts, serum and urine were tested for CP content by enzyme-linked immunosorbent assay (ELISA) and latex agglutination. High concentrations of CP were detected by both assays one-half hour after injection in blood (serum), liver, spleen, kidney and lung (extracts). The duration of ELISA detectable CP was longest (70 days) in liver and spleen and shortest (14 days) in lung extract. By 14 days after injection, concentration of CP in the blood fell below that found in the liver and spleen. CP remained detectable (titers 32-64) after all other extracts became negative. These results indicate that CP is stored in tissues (binding mechanism and site unknown), and that the liver and spleen possess greater storage capacity than other tissues. Antibody (IgM) to CP appeared in low titer on the 14th day and thereafter.
对非免疫小鼠组织和体液中隐球菌多糖（CP）的清除情况进行了研究。仅给小鼠静脉注射1毫克纯化的CP，在84天的时间段内，于不同时间间隔从每只动物获取血清、尿液和组织。通过酶联免疫吸附测定（ELISA）和乳胶凝集试验检测组织提取物、血清和尿液中的CP含量。注射后半小时，在血液（血清）、肝脏、脾脏、肾脏和肺（提取物）中通过两种测定法均检测到高浓度的CP。ELISA可检测到的CP持续时间在肝脏和脾脏中最长（70天），在肺提取物中最短（14天）。注射后14天，血液中CP的浓度降至低于肝脏和脾脏中的浓度。在所有其他提取物变为阴性后，CP仍可检测到（效价为32 - 64）。这些结果表明CP储存在组织中（结合机制和部位未知），并且肝脏和脾脏比其他组织具有更大的储存能力。针对CP的抗体（IgM）在第14天及之后以低滴度出现。
