Buesching W J, Brust R A, Ayers L W
J Clin Microbiol. 1983 Jun;17(6):1153-5. doi: 10.1128/jcm.17.6.1153-1155.1983.
During the 12-month study period, 919 clinical specimens were submitted to the laboratory for culture of Legionella pneumophila. All specimens were plated onto nonselective and semiselective media both directly and after treatment with a KCl-HCl solution, pH 2.2. Of the 51 specimens (5.5%) positive for L. pneumophila and Legionella-like organisms, 20 were recovered by acid pretreatment only, 5 were recovered by direct plating only, and 26 were recovered by both acid pretreatment and direct inoculation. These data demonstrated that acid pretreatment substantially improved the recovery of L. pneumophila and Legionella-like organisms from the clinical specimens examined.
在为期12个月的研究期间，919份临床标本被送到实验室用于嗜肺军团菌培养。所有标本均直接接种以及在经pH值为2.2的氯化钾-盐酸溶液处理后接种到非选择性和半选择性培养基上。在51份（5.5%）对嗜肺军团菌和军团菌样微生物呈阳性的标本中，仅通过酸预处理回收了20份，仅通过直接接种回收了5份，通过酸预处理和直接接种两者回收了26份。这些数据表明，酸预处理显著提高了从所检测的临床标本中回收嗜肺军团菌和军团菌样微生物的效率。
