Biron K K, Noblin J E, de Miranda P, Elion G B
Antimicrob Agents Chemother. 1982 Jan;21(1):44-50. doi: 10.1128/AAC.21.1.44.
The uptake, distribution, and anabolism of the nucleoside analog 9-(2-hydroxyethoxymethyl) guanine (acyclovir) were compared in herpes simplex virus-infected and uninfected mice. Analyses of tissue distribution and the concentration of acyclovir after either a single dose or multiple doses failed to reveal significant differences between drug levels in infected and uninfected animals. Extracts of tissues from [8-14C] acyclovir-treated animals were examined by high-performance liquid chromatography to detect the presence of any phosphorylated forms of the drug. The sensitivity of this method did not allow a reproducible demonstration of acyclovir anabolism in herpes simplex virus-infected tissues owing to the low numbers of infected cells per organ.
在单纯疱疹病毒感染和未感染的小鼠中,对核苷类似物9-(2-羟乙氧甲基)鸟嘌呤(阿昔洛韦)的摄取、分布和合成代谢进行了比较。对单剂量或多剂量给药后阿昔洛韦的组织分布和浓度分析未能揭示感染和未感染动物的药物水平存在显著差异。通过高效液相色谱法检测了用[8-14C]阿昔洛韦处理的动物组织提取物中该药物任何磷酸化形式的存在。由于每个器官中感染细胞数量较少,该方法的灵敏度无法在单纯疱疹病毒感染的组织中重现性地证明阿昔洛韦的合成代谢。