Oken M M, Peterson P K, Wilkinson B J
Infect Immun. 1981 Jan;31(1):208-13. doi: 10.1128/iai.31.1.208-213.1981.
To determine the properties of Staphylococcus aureus contributing to its pyrogenicity, we compared, in human monocytes, endogenous pyrogen production stimulated by heat-killed S. aureus with that stimulated by purified S. aureus cell walls or by particulate peptidoglycan prepared from the same strain. Peptidoglycan, but not the purified cell wall preparation, was found comparable to S. aureus as an endogenous pyrogen stimulus. This finding was associated with a more effective monocyte phagocytosis of S. aureus and peptidoglycan as compared with that of purified cell walls. Lysostaphin digestion of peptidoglycan markedly reduced its pyrogenicity. To test whether the chemical composition of the ingested particles is important, latex particles were tested as possible stimuli for monocyte endogenous pyrogen release. Although 40 to 68% of monocytes ingested latex particles during the first hour, there was no evidence of endogenous pyrogen activity in the supernatant even when supernatants equivalent to 5.2 X 10(6) monocytes were tested. This study demonstrates that the pyrogenic moiety of the S. aureus cell wall resides in the peptidoglycan component. Phagocytosis is not in itself a pyrogenic stimulus, but rather serves as an effective mechanism to bring about contact between the chemical stimulus and the monocyte.
为了确定金黄色葡萄球菌导致其致热原性的特性，我们在人类单核细胞中比较了热灭活的金黄色葡萄球菌、纯化的金黄色葡萄球菌细胞壁或由同一菌株制备的颗粒状肽聚糖刺激产生的内源性致热原。结果发现，肽聚糖作为内源性致热原刺激物与金黄色葡萄球菌相当，而纯化的细胞壁制剂则不然。这一发现与单核细胞对金黄色葡萄球菌和肽聚糖的吞噬作用比纯化细胞壁更有效有关。肽聚糖经溶葡萄球菌酶消化后，其致热原性显著降低。为了测试摄入颗粒的化学成分是否重要，我们测试了乳胶颗粒作为单核细胞内源性致热原释放的可能刺激物。尽管在第一个小时内40%至68%的单核细胞摄入了乳胶颗粒，但即使测试相当于5.2×10⁶个单核细胞的上清液，也没有证据表明上清液中有内源性致热原活性。本研究表明，金黄色葡萄球菌细胞壁的致热部分存在于肽聚糖成分中。吞噬作用本身并不是一种致热刺激，而是一种使化学刺激与单核细胞接触的有效机制。
