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四种革兰氏阴性非发酵菌鉴定方法的比较:临床标本中较少见的微生物

Comparison of four methods for identification of gram-negative non-fermenters: organisms less commonly encountered in clinical specimens.

作者信息

Appelbaum P C, Stavitz J, Bentz M S, von Kuster L C

出版信息

Med Microbiol Immunol. 1981;169(3):163-8. doi: 10.1007/BF02123589.

DOI:10.1007/BF02123589
PMID:7254131
Abstract

Four commercial kits--Oxi/Ferm (OF), API 20E (AP), Minitek (MT), Flow N/F (NF) were evaluated, without additional tests, for identification of 105 opportunistic Gram-negative non-fermentative rods. Of correctly identified 42% of strains, with 35% as part (but not first) of a spectrum of identifications (SI) and 23% incorrect identification. MT yielded 75% correct identification, with 12% SI and 13% incorrect. AP correctly identified 64% of strains, with 26% SI, 10% incorrect. NF correctly speciated 70% of strains, with 24% SI, 6% incorrect. All 4 methods show deficiencies in identification of these rare but increasingly clinically encountered organisms. Addition of new tests/modification of existing ones would render these systems more capable of identifying this organisms group.

摘要

对四种商用试剂盒——Oxi/Ferm(OF)、API 20E(AP)、Minitek(MT)、Flow N/F(NF)进行了评估,无需额外测试,以鉴定105株机会性革兰氏阴性非发酵菌。正确鉴定出42%的菌株,其中35%作为部分(但不是首个)鉴定谱(SI),23%鉴定错误。MT的正确鉴定率为75%,其中12%为SI,13%鉴定错误。AP正确鉴定出64%的菌株,其中26%为SI,10%鉴定错误。NF正确鉴定出70%的菌株,其中24%为SI,6%鉴定错误。所有这四种方法在鉴定这些罕见但临床中越来越常见的微生物时都存在不足。增加新测试/修改现有测试将使这些系统更有能力鉴定这类微生物。

相似文献

1
Comparison of four methods for identification of gram-negative non-fermenters: organisms less commonly encountered in clinical specimens.四种革兰氏阴性非发酵菌鉴定方法的比较:临床标本中较少见的微生物
Med Microbiol Immunol. 1981;169(3):163-8. doi: 10.1007/BF02123589.
2
The N/F and Oxi/Ferm systems for identification of oxidative-fermentative Gram-negative rods: a comparative study.用于鉴定氧化发酵革兰氏阴性杆菌的N/F和Oxi/Ferm系统:一项比较研究。
Acta Pathol Microbiol Immunol Scand B. 1982 Oct;90(5):341-5. doi: 10.1111/j.1699-0463.1982.tb00129.x.
3
Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens.革兰阴性非发酵菌的四种鉴定方法:临床标本中更常见的微生物
J Clin Microbiol. 1980 Aug;12(2):271-8. doi: 10.1128/jcm.12.2.271-278.1980.
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A comparison of four commercial systems for the identification of nonfermentative gram-negative bacilli.四种用于鉴定非发酵革兰氏阴性杆菌的商业系统的比较。
Am J Clin Pathol. 1980 Apr;73(4):564-9. doi: 10.1093/ajcp/73.4.564.
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引用本文的文献

1
Identification of oxidase-positive, glucose-negative, motile species of nonfermentative bacilli.氧化酶阳性、葡萄糖阴性、运动性非发酵杆菌菌种的鉴定。
J Clin Microbiol. 1986 May;23(5):920-3. doi: 10.1128/jcm.23.5.920-923.1986.

本文引用的文献

1
Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens.革兰阴性非发酵菌的四种鉴定方法:临床标本中更常见的微生物
J Clin Microbiol. 1980 Aug;12(2):271-8. doi: 10.1128/jcm.12.2.271-278.1980.
2
Infrequently encountered Pseudomonas species causing infection in humans.在人类中引起感染的罕见假单胞菌属菌种。
Ann Intern Med. 1972 Aug;77(2):211-5. doi: 10.7326/0003-4819-77-2-211.
3
Pseudomonas species in clinical microbiology.临床微生物学中的假单胞菌属
Mt Sinai J Med. 1976 Nov-Dec;43(6):710-26.
4
Comparison of the API 20E and Corning N/F systems for identification of nonfermentative gram-negative rods.用于鉴定非发酵革兰氏阴性杆菌的API 20E系统与康宁N/F系统的比较
J Clin Microbiol. 1979 Aug;10(2):175-9. doi: 10.1128/jcm.10.2.175-179.1979.
5
Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria.API 20E系统与Oxi/Ferm系统在鉴定非发酵菌和氧化酶阳性发酵菌中的比较。
J Clin Microbiol. 1979 Feb;9(2):220-6. doi: 10.1128/jcm.9.2.220-226.1979.
6
Accuracy and reproducibility of the Oxi/Ferm system in identifying a select group of unusual gram-negative bacilli.Oxi/Ferm系统在鉴定一组特定的罕见革兰氏阴性杆菌中的准确性和可重复性。
J Clin Microbiol. 1979 Feb;9(2):180-5. doi: 10.1128/jcm.9.2.180-185.1979.
7
Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria.用于鉴定非发酵革兰氏阴性菌的API 20E系统评估
J Clin Microbiol. 1978 Jun;7(6):539-45. doi: 10.1128/jcm.7.6.539-545.1978.
8
Evaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli.用于鉴定非发酵革兰氏阴性杆菌的Oxi/Ferm管系统的评估
J Clin Microbiol. 1978 Jun;7(6):533-8. doi: 10.1128/jcm.7.6.533-538.1978.
9
Evaluation of two test-kits--API and Oxi Ferm tube--for identification of oxidative-fermentative Gram-negative rods.评估两种检测试剂盒——API和氧化发酵管——用于鉴定氧化发酵革兰氏阴性杆菌。
Med Microbiol Immunol. 1977 Jul 18;163(2):93-7. doi: 10.1007/BF02121824.
10
Rapid identification of nonfermentative gram-negative rods by the Corning N/F system.使用康宁N/F系统快速鉴定非发酵革兰氏阴性杆菌。
J Clin Microbiol. 1979 Feb;9(2):239-43. doi: 10.1128/jcm.9.2.239-243.1979.