Velichko M G, Petushok V G, Gorbach Z V, Trebukhina R V, Ostrovskiĭ Iu M
Vopr Med Khim. 1981 Sep-Oct;27(5):594-600.
Content of lactate in blood, liver and kidney tissues, skeletal muscle and in tumor tissue as well as some properties of lactate dehydrogenase (LDH), isolated from liver tissue, were studied in three groups of rats - intact rats, the tumor-bearing animals with sarcoma S-45 and the tumor-bearing rats treated with hydroxythiamin within 22 days. In skeletal muscle on the side of the tumor localization content of lactate was decreased as compared with the opposite side of the body. As shown by analysis of correlation between the content of lactate and the tumor weight and the lactate concentration in the tumor-bearing rat tissues studied, the tumor appears to be responsible for consumption but not for production of lactate. Hydroxythiamin altered distinctly the ratio of lactate content in various tissues and normalized the liver tissue LDH isoenzyme spectrum in tumor-bearing rats; the vitamin decreased 9- and 15-fold the enzyme specific activity in oxidation of lactate in presence of NAD+ and NADP, respectively. After the hydroxythiamin treatment the apparent KM value of the enzyme, isolated from the tumor-bearing rat liver tissue, was increased with pyruvate and decreased with lactate as substrate.
研究了三组大鼠（正常大鼠、患有肉瘤S - 45的荷瘤动物以及在22天内用羟硫胺治疗的荷瘤大鼠）血液、肝脏、肾脏组织、骨骼肌和肿瘤组织中的乳酸含量，以及从肝脏组织中分离出的乳酸脱氢酶（LDH）的一些特性。与身体另一侧相比，肿瘤定位一侧的骨骼肌中乳酸含量降低。通过对荷瘤大鼠组织中乳酸含量与肿瘤重量以及乳酸浓度之间的相关性分析表明，肿瘤似乎是乳酸的消耗者而非生产者。羟硫胺明显改变了各种组织中乳酸含量的比例，并使荷瘤大鼠肝脏组织的LDH同工酶谱正常化；该维生素分别使在NAD +和NADP存在下乳酸氧化时酶的比活性降低了9倍和15倍。羟硫胺治疗后，从荷瘤大鼠肝脏组织中分离出的酶以丙酮酸为底物时表观KM值增加，以乳酸为底物时表观KM值降低。
