Stoliar O A, Pelley R P, Kaniecki-Green E, Kkaus M H, Carpenter C C
Lancet. 1976 Jun 12;1(7972):1258-61. doi: 10.1016/s0140-6736(76)91735-9.
A pool of colostrum from Guatemalan mothers (Guatemalan colostrum)) obtained 2-4 days post partum inhibited the induced fluid accumulation in rabbit ileal loops when incubated with Vibrio cholerae or Escherichia coli enterotoxin. There was a linear relationship between the quantity of colostrum used and the protection achieved. Pools of Guatemalan breast-milk obtained 15-30 days post partum and North American breast-milk had the same effect when tested with E. coli and V. cholerae enterotoxins, respectively. The antitoxic activity of a given pool correlated with its IgA content but not with the concentration of IgG or IgM. Guatemalan colostrum globulins were precipitated by ammonium sulphate. The globulins were filtered through a 'Biogel A5' column and fractions obtained. When tested in rabbit ileal loops the antienterotoxin activity in these fractions closely paralleled their IgA but not their detectable IgG or IgM content. We hypothesise that IgA antibody to enterotoxin, present in breast-milk of normal mothers, is probably a manifestation of natural immunity. The passive transfer of these antibodies to the infant may explain why breast-milk prevents E. coli diarrhoea in the neonate.
产后2 - 4天采集的危地马拉母亲的初乳样本（危地马拉初乳），与霍乱弧菌或大肠杆菌肠毒素一起孵育时，可抑制兔回肠袢中诱导的液体蓄积。所用初乳的量与所获得的保护作用之间存在线性关系。产后15 - 30天采集的危地马拉母乳样本和北美母乳样本，分别用大肠杆菌和霍乱弧菌肠毒素检测时，具有相同的效果。给定样本的抗毒素活性与其IgA含量相关，而与IgG或IgM的浓度无关。危地马拉初乳球蛋白用硫酸铵沉淀。球蛋白通过“生物凝胶A5”柱过滤并获得各组分。在兔回肠袢中进行检测时，这些组分中的抗肠毒素活性与其IgA含量密切平行，而与其可检测到的IgG或IgM含量无关。我们推测，正常母亲母乳中存在的抗肠毒素IgA抗体可能是自然免疫的一种表现。这些抗体向婴儿的被动转移可能解释了母乳为何能预防新生儿大肠杆菌腹泻。
