Mellbye O J, Fyrand O, Brath H K, Olsen E
Clin Exp Immunol. 1980 Apr;40(1):103-10.
In twelve synovial fluid/serum pairs from patients with various types of seronegative polyarthritis, homogeneous gamma-bands by agarose gel electrophoresis were found in seven of the synovial fluids and in only one of the sera. In six of the fluids with gamma-bands, smooth muscle antibodies (SMA) were also present, usually in a titre identical to that in serum. In fluids with no gamma-bands, no SMA were detected. In forty synovial fluid/serum pairs from paitients with seropositive rheumatoid arthritis, no gamma-bands were detected in the synovial fluids, and SMA were present in only three pairs. Absorption and inhibition experiments did not give evidence that the SMA activity in seronegative polyarthritis was confined to the gamma-bands in the synovial fluids. The SMA activity in the fluids seemed to be directed against both actin and 'non-actin' muscular antigens. The association between locally produced oligoclonal immunoglobulins and possible locally produced SMA with differnet electrophoretic mobility suggests that in some of thes patients there is a local synovial production of oligoclonal antibodies with different specificities. Thus, even if the results may indicate a local virus infection in some arthritic joints, they may also be dur to an unspecific local stimulation of B cells or to a specific antigen stimulation combined with an unspecific co-activation of other antibody-producing cells.
在来自患有各种类型血清阴性多关节炎患者的12对滑液/血清中，通过琼脂糖凝胶电泳在7份滑液中发现了均一的γ带，而血清中仅1份出现γ带。在6份有γ带的滑液中，还存在平滑肌抗体（SMA），其滴度通常与血清中的相同。在没有γ带的滑液中，未检测到SMA。在来自血清阳性类风湿关节炎患者的40对滑液/血清中，滑液中未检测到γ带，仅3对血清中存在SMA。吸收和抑制实验并未证明血清阴性多关节炎中的SMA活性局限于滑液中的γ带。滑液中的SMA活性似乎针对肌动蛋白和“非肌动蛋白”肌肉抗原。局部产生的寡克隆免疫球蛋白与可能具有不同电泳迁移率的局部产生的SMA之间的关联表明，在这些患者中的一些患者中，滑膜局部产生了具有不同特异性的寡克隆抗体。因此，即使结果可能表明某些关节炎关节存在局部病毒感染，它们也可能是由于B细胞的非特异性局部刺激或特异性抗原刺激与其他抗体产生细胞的非特异性共同激活所致。
