Martínez-Martínez L, Suárez A I, Winstanley J, Ortega M C, Bernard K
Department of Microbiology, Hospital Universitario Virgen Macarena, Seville, Spain.
J Clin Microbiol. 1995 Sep;33(9):2458-61. doi: 10.1128/jcm.33.9.2458-2461.1995.
During a 34-month period (January 1991 to October 1993), 31 Corynebacterium striatum stains recovered from clinical samples from 24 patients were characterized. Twenty (64%) strains were isolated from wound exudates, 5 (16%) were isolated from bronchial aspirates, 2 (7%) were isolated from urine, 2 (7%) were isolated from endotracheal tubes, 1 (3%) was isolated from a catheter, and 1 (3%) was isolated from empyema. The organisms were identified by conventional culture and phenotypic characterization, the API CORYNE system, and cellular fatty acid composition analyses. The colonies of C. striatum could be confused with those of coagulase-negative staphylococci upon primary isolation from clinical material. A consistent phenotypic pattern was observed: all strains reduced nitrate, hydrolyzed tyrosine, and produced acid from glucose, fructose, and sucrose but not from maltose. API CORYNE profile numbers were 3100105 (28 strains) and 3000105 (3 strains). Susceptibility testing of C. striatum was performed by disk diffusion. All strains were susceptible to both imipenem and vancomycin and resistant to fosfomycin; most strains were susceptible to ampicillin and cephalosporins and resistant to clindamycin, erythromycin, and tetracycline. Performing a Gram stain of fosfomycin-resistant "Staphylococcus-like" colonies was critical in order to identify C. striatum.
在34个月期间（1991年1月至1993年10月），对从24例患者的临床样本中分离出的31株纹带棒状杆菌菌株进行了特性分析。20株（64%）菌株从伤口渗出物中分离得到，5株（16%）从支气管吸出物中分离得到，2株（7%）从尿液中分离得到，2株（7%）从气管内插管中分离得到，1株（3%）从导管中分离得到，1株（3%）从脓胸中分离得到。通过传统培养和表型特征鉴定、API CORYNE系统以及细胞脂肪酸组成分析对这些微生物进行鉴定。从临床材料初次分离时，纹带棒状杆菌的菌落可能会与凝固酶阴性葡萄球菌的菌落混淆。观察到一种一致的表型模式：所有菌株都能还原硝酸盐、水解酪氨酸，并且能利用葡萄糖、果糖和蔗糖产酸，但不能利用麦芽糖产酸。API CORYNE谱号为3100105（28株）和3000105（3株）。采用纸片扩散法对纹带棒状杆菌进行药敏试验。所有菌株对亚胺培南和万古霉素均敏感，对磷霉素耐药；大多数菌株对氨苄西林和头孢菌素敏感，对克林霉素、红霉素和四环素耐药。对磷霉素耐药的“葡萄球菌样”菌落进行革兰氏染色对于鉴定纹带棒状杆菌至关重要。
