伯氏疏螺旋体ospB基因的变异导致单克隆抗体反应性的差异以及逃逸变异株的产生。
Variations in the ospB gene of Borrelia burgdorferi result in differences in monoclonal antibody reactivity and in production of escape variants.
作者信息
Coleman J L, Rogers R C, Rosa P A, Benach J L
机构信息
State of New York Department of Health, Stony Brook.
出版信息
Infect Immun. 1994 Jan;62(1):303-7. doi: 10.1128/iai.62.1.303-307.1994.
Abstract
An escape variant of Borrelia burgdorferi, selected with a monoclonal antibody to OspB, expressed a truncated form of OspB, the result of point mutations in the ospB gene leading to a premature termination codon. A single amino acid position in the C terminus of OspB was critical for monoclonal antibody recognition. The variations in the ospB gene suggest a mechanism for the evasion of the immune response by these organisms and may also have implications for current diagnostic and vaccine efforts.
摘要
用针对OspB的单克隆抗体筛选出的伯氏疏螺旋体逃逸变异株表达了截短形式的OspB，这是ospB基因中的点突变导致提前终止密码子的结果。OspB C末端的单个氨基酸位置对单克隆抗体识别至关重要。ospB基因的变异提示了这些病原体逃避免疫反应的一种机制，也可能对当前的诊断和疫苗研发工作产生影响。
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