Reina J, Ros M J, Serra A
Clinical Microbiology Service, University Hospital Son Dureta (UIB), Palma de Mallorca, Spain.
J Clin Pathol. 1995 Jul;48(7):683-5. doi: 10.1136/jcp.48.7.683.
The aim was to evaluate the efficacy of the API-Campy system in the biochemical identification of 62 hippurate negative campylobacter strains isolated from the faeces. The strains were identified manually as 34 nalidixic acid susceptible C coli (NAS), 20 nalidixic acid resistant C coli (NAR), and eight C lari. The 34 strains of NAS C coli were identified as such by the API-Campy system. Of the 20 strains of NAR C coli, 15 (75%) were correctly identified by the commercial system. None of the five NAR C coli strains which were also erythromycin resistant was identified as such by the system. The eight C lari strains could not be identified by the API-Campy system because the bionumber obtained does not exist in the database of the computer system. The API-Campy system could be very useful for the identification of NAS C coli. However, failure to allow for a higher percentage of resistance to nalidixic acid in this species does not permit good identification of NAR strains. More important discrepancies are observed in C lari strains. In order to improve the identification of NAR C coli and C lari stains, it is advisable to include, or recommend as complementary, the indoxyl acetate hydrolysis test.
目的是评估API - Campy系统对从粪便中分离出的62株马尿酸盐阴性弯曲杆菌菌株进行生化鉴定的效果。这些菌株经人工鉴定为34株对萘啶酸敏感的大肠弯曲菌（NAS）、20株对萘啶酸耐药的大肠弯曲菌（NAR）和8株拉氏弯曲菌。34株NAS大肠弯曲菌经API - Campy系统鉴定为该菌。在20株NAR大肠弯曲菌中，15株（75%）被该商业系统正确鉴定。5株同时对红霉素耐药的NAR大肠弯曲菌菌株均未被该系统鉴定出来。8株拉氏弯曲菌菌株无法用API - Campy系统鉴定，因为计算机系统数据库中不存在所获得的生物编号。API - Campy系统对NAS大肠弯曲菌的鉴定可能非常有用。然而，该系统未考虑到该菌种中对萘啶酸耐药比例较高的情况，因此无法很好地鉴定NAR菌株。在拉氏弯曲菌菌株中观察到更严重的差异。为了提高对NAR大肠弯曲菌和拉氏弯曲菌菌株的鉴定，建议纳入或推荐使用醋酸吲哚酚水解试验作为补充试验。
