Buckley T J, Waldman J M, Dhara R, Greenberg A, Ouyang Z, Lioy P J
Department of Environmental Science, Rutgers University, Piscataway, NJ, USA.
Int Arch Occup Environ Health. 1995;67(4):257-66. doi: 10.1007/BF00409408.
Urinary banzo[a]pyrene (BaP) metabolite levels were compared to human environmental exposure to BaP through inhalation and dietary ingestion to assess the predictive validity of the exposure biomarker. These measurements were made for 14 adult volunteers over 14 consecutive days, once during summer/fall, again during winter periods. Based on personal air monitoring, median potential inhalation doses of 11.0 and 2.3 ng/day were estimated for the winter and summer/fall studies, respectively. A median potential ingested dose of 176 ng/day, estimated from "duplicate plate" sampling, exceeded inhalation by 6- and 122-fold for the winter and summer/fall studies, respectively. "Total" urinary BaP metabolites were measured using a published "reverse metabolism" (BaP) method of analysis. Median rates of urinary BaP metabolite elimination for the winter and summer/fall studies were 121 and 129 ng/day, respectively. The changes in inhaled and ingested potential doses were regressed on the change in urinary metabolite elimination from week 1 to week 2 to test the predictive validity of the biomarker measurement. The regression was statistically significant (r = 0.620, p = 0.015, n = 25) when body weight was included and two extreme values were removed. Consistent with the exposure measurements showing diet as the dominant route of exposure, most of the variation in urinary metabolite elimination was explained by the ingested dose. It is concluded that the measurement of urinary BaP by "reverse metabolism" is qualitative and of marginal predictive validity as an exposure biomarker due to the method's low recoveries and the large unexplained variance.
将尿中苯并[a]芘（BaP）代谢物水平与人类通过吸入和饮食摄入而接触的环境BaP进行比较，以评估该接触生物标志物的预测效度。对14名成年志愿者连续14天进行了这些测量，一次在夏季/秋季，一次在冬季。根据个人空气监测，冬季和夏季/秋季研究中估计的潜在吸入剂量中位数分别为11.0和2.3纳克/天。通过“双份餐盘”采样估计的摄入剂量中位数为176纳克/天，在冬季和夏季/秋季研究中分别比吸入量高6倍和122倍。使用已发表的“反向代谢”（BaP）分析方法测量“总”尿BaP代谢物。冬季和夏季/秋季研究中尿BaP代谢物消除的中位数速率分别为121和129纳克/天。将吸入和摄入的潜在剂量变化与第1周和第2周尿代谢物消除变化进行回归分析，以测试生物标志物测量的预测效度。当纳入体重并去除两个极端值时，回归具有统计学意义（r = 0.620，p = 0.015，n = 25）。与显示饮食是主要接触途径的接触测量结果一致，尿代谢物消除的大部分变化可以由摄入剂量来解释。结论是，由于该方法回收率低且存在大量无法解释的差异，通过“反向代谢”测量尿BaP作为接触生物标志物具有定性性质且预测效度有限。
