Roberts J A, Kaack M B, Martin L N
Department of Urology, Tulane Regional Primate Research Center, Covington, LA 70433, USA.
Urol Res. 1995;23(1):33-8. doi: 10.1007/BF00298848.
We studied the cellular and humoral events which follow experimental acute pyelonephritis from P-fimbriated Escherichia coli to gain insight into the relationships among cells and specifically cytokines to determine how early events in untreated infection lead to renal damage. Cynomolgus (Macaca fascicularis) monkeys were studied after they were subjected to unilateral ureteral bacterial inoculation. We evaluated the blood for leukocytosis and studied lymphocyte subsets using flow cytometry and monoclonal antibodies to the subsets and serum, complement, cytokines and antibody titers. Interleukin-1, 2 and 6 and tumor necrosis factor (TNF) were assayed by enzyme-linked immunoadsorbent assay (ELISA), using monoclonal and polyclonal antibodies. Leukocytosis was marked and there were significant elevations in serum cytokines, interleukin-1 alpha, 2 and 6 with only small changes in the level of TNF. Interleukin-2 levels were sustained and may have upregulated the homing receptor for virgin lymphocytes. The studies illustrated the unique relationship between cytokines and lymphocytes and the response to bacterial infection, showing that the inflammatory response is regulated not only by cytokine activity but also by lymphocyte activation.
我们研究了实验性急性肾盂肾炎（由产P菌毛的大肠杆菌引起）后的细胞和体液事件，以深入了解细胞间的关系，特别是细胞因子之间的关系，从而确定未经治疗的感染早期事件如何导致肾损伤。对食蟹猴（猕猴）进行单侧输尿管细菌接种后展开研究。我们评估血液中的白细胞增多情况，并使用流式细胞术以及针对淋巴细胞亚群、血清、补体、细胞因子和抗体滴度的单克隆抗体来研究淋巴细胞亚群。使用单克隆抗体和多克隆抗体通过酶联免疫吸附测定（ELISA）法检测白细胞介素-1、2和6以及肿瘤坏死因子（TNF）。白细胞增多明显，血清细胞因子、白细胞介素-1α、2和6显著升高，而TNF水平仅有微小变化。白细胞介素-2水平持续存在，可能上调了初始淋巴细胞的归巢受体。这些研究阐明了细胞因子与淋巴细胞之间的独特关系以及对细菌感染的反应，表明炎症反应不仅受细胞因子活性调控，还受淋巴细胞激活的影响。
