Konno A, Hashimoto Y, Kon Y, Sugimura M
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Vet Med Sci. 1994 Dec;56(6):1101-5. doi: 10.1292/jvms.56.1101.
Distributional and immunohistochemical characteristics of the feline globule leukocyte (GL) were investigated by light microscopy. The GL, which contained eosinophilic large granules in the cytoplasm, was frequently found in the epithelia of the intestine and gall bladder, and less frequently found in those of the gastric pit, intrahepatic bile duct, and interlobular secretory duct of the pancreas. No GL was seen in the respiratory and urogenital organs. The GLs composed a homogeneous cell population including no mast cells according to the following histochemical stainings; phosphotungstic acid hematoxylin, alcian blue and peroxidase. The feline GL showed perforin-like immunoreactivity to anti-human perforin monoclonal antibody, but did not show histamine-immunoreactivity to anti-histamine polyclonal antibody. The results suggest that the feline GL is a lineage of large granular lymphocytes. The epitheliotropism and characteristics as granular lymphocytes of the feline GLs were similar to those of the intestinal gamma delta T cells of the mouse.
通过光学显微镜研究了猫球状白细胞（GL）的分布和免疫组织化学特征。GL的细胞质中含有嗜酸性大颗粒，常见于肠道和胆囊上皮，较少见于胃小凹、肝内胆管和胰腺小叶间分泌管上皮。在呼吸和泌尿生殖器官中未见GL。根据以下组织化学染色，GL构成了一个不包括肥大细胞的同质细胞群；磷钨酸苏木精、阿尔辛蓝和过氧化物酶。猫GL对抗人穿孔素单克隆抗体表现出穿孔素样免疫反应，但对抗组胺多克隆抗体未表现出组胺免疫反应。结果表明，猫GL是大颗粒淋巴细胞的一个谱系。猫GL的上皮嗜性和作为颗粒淋巴细胞的特征与小鼠肠道γδT细胞相似。
