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用抗γFc吸收后全血清中IgM风疹抗体的免疫荧光试验

Immunofluorescence test for IgM rubella antibodies in whole serum after absorption with anti-gammaFc.

作者信息

Gispen R, Nagel J, Brand-Saathof B, De Graaf S

出版信息

Clin Exp Immunol. 1975 Dec;22(3):431-7.

Abstract

A technique for detecting IgM rubella antibodies by immunofluorescence was developed. Total serum IgG was removed by prior immunoabsorption with anti-gammaFc. This prevents interference by rheumatoid factors in the serum. The test appeared sensitive and specific in sera of rubella convalescents and infants with congenital rubella.

摘要

开发了一种通过免疫荧光检测IgM风疹抗体的技术。通过用抗γFc进行预先免疫吸附去除血清中的总IgG。这可防止血清中类风湿因子的干扰。该试验在风疹恢复期患者和先天性风疹婴儿的血清中显示出敏感性和特异性。

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本文引用的文献

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Rubella-virus hemagglutination-inhibition test.风疹病毒血凝抑制试验
N Engl J Med. 1967 Mar 9;276(10):554-7. doi: 10.1056/NEJM196703092761006.
4
The IgM antibody response in rubella during pregnancy.孕期风疹中的IgM抗体反应。
J Med Microbiol. 1971 Feb;4(1):107-14. doi: 10.1099/00222615-4-1-107.
8
Fluorescent staining and human IgM.荧光染色与人类免疫球蛋白M
Br Med J. 1971 Sep 18;3(5776):707. doi: 10.1136/bmj.3.5776.707.

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